These results may also be relevant.
-
p3xFLAG-Tmprss6(S762A)
Plasmid#18791DepositorInsertType II transmembrane serine protease 6 (Tmprss6 Mouse)
UseTags3xFLAGExpressionMammalianMutationChanged Serine762 to AlaninePromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ
Plasmid#51495PurposepLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmidDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant t-dna plasmidTagsSV40 NLSExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNAM-B1
Plasmid#154056PurposeLevel 0 Golden Gate vector, contains domesticated NAM-B1DepositorInsertTaNAM-B1
UseSynthetic BiologyTagsExpressionBacterialMutationSynonymous mutation at base 3257, in exon 3, to r…PromoterN/AAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
-
pGFP-Tmprss6 (delC)
Plasmid#18793DepositorInsertType II transmembrane serine protease 6 (Tmprss6 Mouse)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 81-811PromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Tmprss6 (delN)
Plasmid#18792DepositorInsertType II transmembrane serine protease 6 (Tmprss6 Mouse)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-54PromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
ecALDH
Plasmid#67921PurposeExpresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coliDepositorInsertEscherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
EMSV-Myf5 (puro)
Plasmid#14711DepositorInsertMyf5 (Myf5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
pBa.GFP-KIF13B
Plasmid#134626PurposeMammalian expression of mouse KIF13B 442-1843DepositorInsertKIF13B (Kif13b Mouse)
UseTagseGFPExpressionMammalianMutationWTPromoterChicken Beta actinAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9-GFP_sg_mAMPKa1
Plasmid#79004PurposeExpresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.DepositorInsertPrkaa1 (Prkaa1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTpal-fdc
Plasmid#78288PurposeExpresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coliDepositorUseTagsExpressionBacterialMutation*see belowPromotertrcAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
EC71100
Plasmid#154068PurposeLevel 0 Golden Gate vector, pHvHSP17DepositorInsertpHvHSP17
UseStandard cloning vector used by geneart for synth…TagsExpressionMutationAll BsaI, Esp3I and BpiI restriction sites were r…PromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC71022
Plasmid#159608PurposeLevel 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxPDepositorInsertloxP-mCherry-HDEL-t35S-loxP
UseCre/Lox and Synthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myo1E-pEGFP
Plasmid#124658PurposeMouse myosin-1e fused with EGFPDepositorInsertMyo1e (Myo1e Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:TpCC_Urease
Plasmid#85982PurposeGolden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAsDepositorInsertsCas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2
UseCRISPR; Diatom (t. pseudonana)TagsNLS and YFPExpressionMutationPromoterFCP and U6Available sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b SARS-CoV PLpro
Plasmid#61693Purposeinducible expression of SARS PLpro proteaseDepositorInsertSARS-CoV PLpro
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
AMPK-β1-FLAG
Plasmid#40602DepositorInsertAMPK-β1 (Prkab1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only
