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Plasmid#73839PurposeProduces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT5-TDH3p-ycrtYB-ADH1t
Plasmid#65358PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTDH3p-ycrtYB-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT2-TDH3p-ycrtE-ADH1t
Plasmid#65352PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTDH3p-ycrtE-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS2-aurZ
Plasmid#70151PurposeFusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertaurZ
UseTagsExpressionYeastMutationPromoterTefAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS3-aurJ
Plasmid#70152PurposeFusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection markerDepositorInsertaurJ
UseTagsExpressionYeastMutationPromoterGPD1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS2
Plasmid#70135PurposeCEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertDS2
UseSynthetic BiologyTagsExpressionMutationPromoterAgTEF1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Gm1
Plasmid#53528PurposeExpresses a plant arginyl-tRNA synthetase in E.coliDepositorInsertarginyl-tRNA synthetase
UseTagsHis Tag and ThioredoxinExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pRSET FLII282Pglu-4m
Plasmid#13564DepositorInsertFLIPglu F16A T282S-ECFP-N283R
UseTagsCitrineExpressionBacterialMutationF16A, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBbE8k-fcs
Plasmid#83068PurposeExpresses Fcs under pBAD regulationDepositorInsertfcs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cre.zf1
Plasmid#61391PurposemRNA synthesis of Cre recombinase codon modified for zebrafishDepositorInsertCre.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-puro
Plasmid#125839Purposemodifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
MCS-BioID2-HA
Plasmid#74224PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
myc-BioID2-MCS
Plasmid#74223PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsMyc and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-PPARA
Plasmid#169019PurposeExpression of PPARA driven by CMV promoterDepositorInserthuman ppara (PPARA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Renilla_Luciferase
Plasmid#74444PurposeControl lentiviral vector expressing Renilla luciferaseDepositorInsertRenilla Luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidTagsExpressionBacterialMutationPromoterpTrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSerOTS-C1* (V70)
Plasmid#188537PurposePhosphoserine incorporation into recombinant proteinsDepositorInsertspSerRS
Ef-pSer
tRNApSer
UseTagsExpressionBacterialMutationmutations resulting from laboratory evolutionPromoterglnS*Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-OMMjRCaMP-P2A-mito4xGCaMP6f
Plasmid#127872PurposeDual expression vector for mitochondrial matrix and outer membrane Ca2+ sensorsDepositorInsertOuter membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Tol1.zf1
Plasmid#61388PurposemRNA synthesis of tol1 transposase codon modified for zebrafishDepositorInserttol1.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-LAMP1-RFP-3xHA
Plasmid#102932PurposeExpress a lysosomal TagDepositorInsertLAMP1
UseLentiviralTagsRFP-HAExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shSHMT2
Plasmid#106319PurposeExpress shRNA targeting SHMT2DepositorInsertshRNA targeting SHMT2
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb-IRES-eGFP-W
Plasmid#32519DepositorInsertRheb (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-LAMP1-RFP-2xFlag
Plasmid#102931PurposeExpress a lysosomal TagDepositorInsertLAMP1
UseLentiviralTagsmRFP-2xFlagExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec633
Plasmid#130873PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationL477V, L640Y, I777K, W814NPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-FLAG-mPKM2
Plasmid#44239DepositorInsertPyruvate Kinase M2 (Pkm Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mem
Plasmid#55779PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.DepositorInsertmCherry-Mem
UseTagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHBintE
Plasmid#48136PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCas2C
Plasmid#82374PurposedCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas34
Plasmid#82386PurposesgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec611
Plasmid#130872PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationI777K, L900SPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV
Plasmid#202004PurposeEmpty lenti backboneDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-eGFP
Plasmid#32548DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGAN230
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK221
Plasmid#129249Purposereverse yTRAP, tetR-responsive Neon reporterDepositorInsertpADH1(tetO2)-mNeonGreen
UseTagsExpressionYeastMutationPromoterADH1Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5-FRT-TO-N-Flag-8His
Plasmid#157677PurposeN-terminal tagging vectorDepositorInsertFlag-8His
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Salsa-BC1-P1A1
Plasmid#202842PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1DepositorInsertEGFP/BC1
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only