Addgene

We narrowed to 17,069 results for: gateway

Showing: 6741 - 6760 of 17069 results

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pDONR221 Ub-NLRP1 S1213 P1214R

Plasmid

#166830

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Ubiquitin

Expression

Mutation

P1214R

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala271

Plasmid

#158631

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

Q271A please see depositor comments below

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Gln93

Plasmid

#158629

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

E93Q

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Gln93,Ala271

Plasmid

#158632

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

E93Q/Q271A

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala148,Ala271

Plasmid

#158633

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

Q148A/Q271A

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala148

Plasmid

#158630

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

Q148A

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-SERCA1

Plasmid

#109552

Purpose

Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

sarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 allKR

Plasmid

#129298

Purpose

Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed all 22 Lysine residues to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 K295R

Plasmid

#129302

Purpose

Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 295 to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

Plasmid

#121840

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-EGFP L1-R5

Plasmid

#84391

Purpose

Multisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-S37A-GATA6-3XAU1

Plasmid

#72613

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Mutation

Changed serine37 to alanine

Promoter

TRE tight promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2c-E471Q

Plasmid

#45704

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

E471Q

Promoter

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00698

Plasmid

#39735

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8599 (Su(var)3-7 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00290

Plasmid

#34416

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7771 (sim Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00320

Plasmid

#34445

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6376 (E2f1 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00321

Plasmid

#34446

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8676 (Hr39 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00205

Plasmid

#34356

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8068 (Su(var)2-10 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00050

Plasmid

#34212

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11502 (svp Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00012

Plasmid

#34174

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8328 (HLHmdelta Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only