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Plasmid#166830PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsUbiquitinExpressionMutationP1214RPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala271
Plasmid#158631PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ271A please see depositor comments belowPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Gln93
Plasmid#158629PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationE93QPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Gln93,Ala271
Plasmid#158632PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationE93Q/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala148,Ala271
Plasmid#158633PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ148A/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala148
Plasmid#158630PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ148APromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-SERCA1
Plasmid#109552PurposeMultisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1DepositorInsertsarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 allKR
Plasmid#129298PurposeExpresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged all 22 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 K295R
Plasmid#129302PurposeExpresses pCMV 3xFLAG-ATG3 K295R in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 295 to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L
Plasmid#121840PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-EGFP L1-R5
Plasmid#84391PurposeMultisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsEGFPExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-S37A-GATA6-3XAU1
Plasmid#72613PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationChanged serine37 to alaninePromoterTRE tight promoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2c-E471Q
Plasmid#45704DepositorInsertFGFR2 IIIc (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationE471QPromoterAvailable sinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
KHBD00698
Plasmid#39735DepositorInsertCG8599 (Su(var)3-7 Fly)
UseGateway donr vectorTagsExpressionMutationPromoternoneAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
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KHBD00205
Plasmid#34356DepositorInsertCG8068 (Su(var)2-10 Fly)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
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