We narrowed to 16,094 results for: LEA

1. SpCas9-NRCH-CGBE1

   Plasmid

   #198557

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-CGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRoC30_Th3.1

   Plasmid

   #188065

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJRoC30_Th3.7

   Plasmid

   #188066

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 3 FuncLib mutations, described in publ…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRoC30_Th3.10

   Plasmid

   #188067

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 3 FuncLib mutations, described in publ…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM-U6-reRNA-mmPCSK9

   Plasmid

   #205634

   Purpose

   reRNA guide targeting Mus Musculus PCSK9

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-mmPCSK9 (Pcsk9 Mouse)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPSt_35Spr-GFP-t35S

   Plasmid

   #203592

   Purpose

   Fully assembled Plant STARR-seq plasmid with the 35S terminator.

   Depositor

   Stan Fields

   Article

   Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

   Inserts

   CaMV 35S promoter + Zm00001d041672 5'UTR
   eGFP
   CaMV 35S terminator
   

   Expression

   Plant

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

   Plasmid

   #215699

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-EF1a-ArgiNLS-miRFP670

   Plasmid

   #220600

   Purpose

   Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   miRFP670
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

   Plasmid

   #220597

   Purpose

   Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mVenus-Q69M (ME)
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-ArgiNLS-mKate2

    Plasmid

    #220599

    Purpose

    Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-FLEX-ArgiNLS-EGFP

    Plasmid

    #220601

    Purpose

    Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

    Plasmid

    #195018

    Purpose

    An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

    Depositor

    Christopher Richie

    Article

    Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

    Inserts

    CCGTATAGGCGGCTGCCCAA
    EGFP-KASH
    TTCCGTGGTGGGCAACGTAG
    

    Use

    AAV

    Tags

    KASH domain of Nesprin2

    Promoter

    EF1a, hU6, and mU6

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSAVED-CHAT

    Plasmid

    #214041

    Purpose

    Bacterial expression plasmid for SAVED-CHAT from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. KU70-eGFP-SUN1ΔN

    Plasmid

    #213516

    Purpose

    Expresses human KU70-eGFP-SUN1ΔN in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    KU70-eGFP-SUNΔN
    

    Expression

    Mammalian

    Mutation

    SUN1ΔN has the first 138 amino acid (lamina domai…

    Promoter

    CMV

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. DRD2-V2tail-TevN-BLITz-TetR-FKBP

    Plasmid

    #210514

    Purpose

    expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    DRD2-V2tail-TevN-BLITz-TetR-FKBP
    

    Tags

    HA signal FALG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. WT_IFL_G1A-T2A-A28C_R

    Plasmid

    #200185

    Purpose

    WT PA14 I-F Leader and G1A-T2A-A28C Repeat

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
    

    Expression

    Bacterial

    Mutation

    G1A-T2A-A28C in the I-F repeat

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-GRAB_g5-HT2h

    Plasmid

    #208715

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT2h
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFSynW SYT9 D197, 199N IRES GFP

    Plasmid

    #195701

    Purpose

    Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Mutation

    full-length mouse SYT9 with D197, 199N mutations

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFSynW SYT9 D330, 332N IRES GFP

    Plasmid

    #195702

    Purpose

    Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Mutation

    full-length mouse SYT9 with D330, 332N mutations

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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