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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,702 results for: LEA

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Showing: 6741 - 6760 of 24702 results

  5. pRK5_tAKAR4α

    Plasmid
    #119919
    Purpose
    Mammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging
    Depositor
    Haining Zhong
    Publication
    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tAKAR4α
    Expression
    Mammalian
    Available since
    March 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119919-plasmid-map-sequence-id-235218

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  8. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

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  9. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

    Plasmid
    #134653
    Purpose
    lysosome-targeted Sac1 catalytic domain C389S
    Depositor
    Roberto Zoncu
    Publication
    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218
    Insert
    Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
    Use
    Lentiviral
    Tags
    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…
    Expression
    Mammalian
    Mutation
    C389S and contains only amino acids 77-520 (Pleas…
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134653-plasmid-map-sequence-id-266494

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  16. pET23a_mod_HuD_FL

    Plasmid
    #65751
    Purpose
    plasmid for bacterial overexpression of HuD
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Tags
    6X His and FLAG HA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65751-plasmid-map-sequence-id-114929

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  17. pTALEN(Nd154C63) SFFV

    Plasmid
    #50431
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50431-plasmid-map-sequence-id-75352

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  18. pDN-D2irTN6kwh

    Plasmid
    #44724
    Depositor
    Gabor Balazsi
    Publication
    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pCMV-D2i promoter
    htetR::NLS
    Use
    Synthetic Biology; Expression regulator/reporter;…
    Tags
    Rabbit β-globin intron II and WPRE
    Expression
    Mammalian
    Mutation
    Initiator motif (Inr) displaced relative to pCMV-…
    Promoter
    pCMV-D2i
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    44724-plasmid-map-sequence-id-61266

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Showing: 6741 - 6760 of 24702 results