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Plasmid#172471PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1.DepositorInsertsgAgo1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SPSB1_SPRY
Plasmid#178487PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertSPSB1_SPRY (SPSB1 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_1-440-V5His6_D
Plasmid#146123PurposeInsect Expression of DmEDC3_1-440DepositorInsertDmEDC3_1-440 (Edc3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG.INBox-mCherry-INBox-eDHFR
Plasmid#176203PurposeDouble INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization systemDepositorInsertINBox-mCherry-INBox-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_APV-SBP-Dendra2-ER-5
Plasmid#170679PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.DepositorInsertsAPV-SBP-Dendra2-ER-5
streptavidin-KDEL
UseTagsExpressionMammalianMutationPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PB1-AG-FRB-TEVuncs-mTagBFP2-HA
Plasmid#178869PurposeMammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVuncs-mTagBFP-HADepositorInsertPB1-AzamiGreen-FRB-TEVuncs(NEYLQFL)-mTagBFP2-HA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mCherry-Zdk1-Vav2cat
Plasmid#178875PurposeMammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1DepositorInsertmCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-INBox-eDHFR
Plasmid#176202PurposeSingle INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization systemDepositorInsertmCherry-INBox-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-INBox(AAA)-eDHFR
Plasmid#176207PurposeINBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization systemDepositorInsertmCherry-INBoxAAA-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlY282F
Plasmid#172468Purposeexpresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domainDepositorInsertwbp2nlY282F
UseXenopus expressionTagsnoneExpressionMutationchanged Tyrosine at aa282 to PhenylalaninePromoterSP6Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174528PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertPylS
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-197
Plasmid#169924PurposeWT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2DepositorInsertEcm2 1-197
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hNKD2
Plasmid#174329Purposeencodes human NKD2 cDNA with a C-terminal MycHis Tag.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsMycHisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH776_pJH_LldR_PlldR-sfGFP.3opal
Plasmid#163735PurposeLactate-inducible expression of green fluorescent protein encoding three opal codonsDepositorInsertlldr gfp.3TGA
UseTagsExpressionBacterialMutationgfp with first three leucine codons mutated to am…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH775_pJH_LldR_PlldR-sfGFP.2opal
Plasmid#163734PurposeLactate-inducible expression of green fluorescent protein encoding two opal codonsDepositorInsertlldr gfp.2TGA
UseTagsExpressionBacterialMutationgfp with first two leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlF127P
Plasmid#174507Purposeexpresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domainDepositorInsertwbp2nlF127P
UseXenopus expressionTagsnoneExpressionMutationchanged Phenylalanine at aa127 to ProlinePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlT45A
Plasmid#172464Purposeexpresses protein with one aa substitution to affect potential phosphorylationDepositorInsertwbp2nlT45A
UseXenopus expressionTagsnoneExpressionMutationchanged Threonine at aa45 to AlaninePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlY55F
Plasmid#172466Purposeexpresses protein with one aa substitution to affect potential phosphorylation in ITSM domainDepositorInsertwbp2nlY55F
UseXenopus expressionTagsnoneExpressionMutationchanged Tyrosine at aa55 to PhenylalaninePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AraC-pHG165c WT
Plasmid#170114PurposeConstitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.DepositorInsertAraC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY156: SUZ12 px330 sgRNA Cas9 plasmid
Plasmid#170792PurposeThis plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153DepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only