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Plasmid#182125PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc LbaCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a
Plasmid#182126PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
Plasmid#182121PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
Plasmid#182124PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.NWS_mCherry-NLS
Plasmid#178282PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.NWS and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIS_TEV_hsSYX3
Plasmid#179290PurposeE. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7lacAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Arg57Ala
Plasmid#180759PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)DepositorInsertα-COPI-WD40-Arg57Ala
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Arg57Ala, Leu1…PromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Tyr139Ala
Plasmid#180761PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)DepositorInsertα-COPI-WD40-Tyr139Ala
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Tyr139Ala, Leu…PromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Asp115Ala
Plasmid#180760PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)DepositorInsertα-COPI-WD40-Asp115Ala
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Asp115Ala, Leu…PromoterCMVAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Chpt1
Plasmid#175155PurposeLentiviral expression of V5-tagged mouse Chpt1DepositorInsertChpt1 (Chpt1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_NWS.S.V5_mCherry-NLS
Plasmid#178281PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsNWS.S.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_NWS.Ollas.V5_mCherry-NLS
Plasmid#178280PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsNWS.Ollas.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HSV.NWS.V5_mCherry-NLS
Plasmid#178275PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHSV.NWS.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HSV.NWS.VSVg_mCherry-NLS
Plasmid#178274PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHSV.NWS.VSVg and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HSV.NWS.Ollas_mCherry-NLS
Plasmid#178272PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHSV.NWS.Ollas and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.VSVg.V5_mCherry-NLS
Plasmid#178271PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHA.VSVg.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.S.V5_mCherry-NLS
Plasmid#178270PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHA.S.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.Ollas.V5_mCherry-NLS
Plasmid#178268PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHA.Ollas.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.Ollas.VSVg_mCherry-NLS
Plasmid#178267PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHA.Ollas.VSVg and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.Ollas.S_mCherry-NLS
Plasmid#178266PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHA.Ollas.S and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only