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pAGM51559

Plasmid

#153226

Purpose

Control plasmid for knockout of TRY and CPC genes

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Cas9, guide RNA for AP3, FAST marker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBT346_pLambda-attB-pCA-GFP-pA

Plasmid

#52551

Purpose

for producing PhiC31 attB-containing minicircle in vitro

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

Lambda integrase attL1
Lambda integrase attR1
PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pJRA108

Plasmid

#209341

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

Plasmid

#128608

Purpose

Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin S3A

Plasmid

#163604

Purpose

Expresses constitutively active cofilin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S3A substitution to make cofilin constitutively a…

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJRA107

Plasmid

#209340

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Lck-FRB-mTurquoise2

Plasmid

#176130

Purpose

Plasma membrane localized FRB, rapamycin heterodimerization

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

FRB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-QUASM1

Plasmid

#155114

Purpose

5' entry vector containing QUASM1 promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1

Use

Gateway 5' entry vector

Tags

Expression

Mutation

Promoter

QUASM1-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-Smad4-Flag

Plasmid

#37041

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

Insert

Smad4 (SMAD4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only
pQCascade(Ptr)-entry(BsaI)

Plasmid

#175579

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-IREShygro2

Plasmid

#153966

Purpose

Expresses EGFP in mammalian cells

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

EGFP

Use

Tags

N/A

Expression

Mammalian

Mutation

No mutation

Promoter

CMV promoter

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-TKBsd-L2

Plasmid

#141009

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

TKBsd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTetQCas-BsaI

Plasmid

#170629

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-IRES-ERT2CreERT2-IRES-Hyg

Plasmid

#159927

Purpose

Gene targeting

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

Insert

ERT2CreERT2

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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SAFB1-pcDNA3.1

Plasmid

#32742

Depositor

Steffi Oesterreich

Article

Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

Insert

SAFB1 (SAFB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Sema4D

Plasmid

#51599

Purpose

expresses mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Use

Tags

myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pGEX2TK-Osh2-PH

Plasmid

#71370

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Use

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Promoter

tac

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pKPY514

Plasmid

#62598

Purpose

Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr251Gly-EcPheRS

Use

Tags

6xHis

Expression

Bacterial

Mutation

Thr251Gly

Promoter

T5

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP

Plasmid

#64111

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX2TK-Num1-PH

Plasmid

#71369

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Use

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Promoter

tac

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only