Addgene

We narrowed to 19,042 results for: tes

Showing: 6701 - 6720 of 19042 results

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Phat2-hs-PKCgamma-C2-EGFP-6H

Plasmid

#187115

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000126583 (PRKCG Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H

Plasmid

#187187

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

D34K, D87K

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

Plasmid

#187186

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Y90F, V91F

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1-dead

Plasmid

#185572

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1-dead (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Expression

Mutation

K82R kinase-dead mutant

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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FAK C-term

Plasmid

#186147

Purpose

For expresseion of recombinant FAK c-terminal domain in E. coli

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-Control (pSLIK3)

Plasmid

#113858

Purpose

An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

E2-Crimson

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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TRE-RARa-VP16

Plasmid

#185561

Purpose

Doxycycline inducible expression of constitutively active Retinoic Acid Receptor alpha

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

RARa-VP16 (Rara Mouse)

Use

Tags

VP16

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Arkadia

Plasmid

#180372

Purpose

expressing mouse Arkadia proteins

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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p8291 MSCV-Neo C-HA 18E7 ΔDLLC

Plasmid

#184523

Purpose

Expression of HPV18 E7 ΔDLLC

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

HPV18 E7 ΔDLLC

Use

Retroviral

Tags

HA

Expression

Mutation

deletion of DLLC

Promoter

MSCV LTR

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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p8319 pDONR ATG-YAP1 WT-Stop

Plasmid

#184524

Purpose

Subcloning YAP1 WT

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 WT (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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NK85

Plasmid

#176611

Purpose

To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

SNAP-FCERG-mGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

C25A

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-HsTNRC6A_L

Plasmid

#146843

Purpose

Insect Expression of HsTNRC6A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A (TNRC6A Human)

Use

Tags

Expression

Insect

Mutation

A339T, K1578E compared to the sequence of isoform…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 1

Plasmid

#154253

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 1 of New World monkey retroCHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 2

Plasmid

#154254

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 2 of New World monkey retroCHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 3

Plasmid

#154255

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 3 of New World monkey retroCHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-5'UTR(mtIF3)-Dendra2-3'UTR(mtIF3)

Plasmid

#182369

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Use

Tags

Pal-Dendra2

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1

Plasmid

#182376

Purpose

Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

3xFlag
mito-mTFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter and SV40 promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-DmRoquin_AD

Plasmid

#148531

Purpose

Mammalian Expression of DmRoquin

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin (roq Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_1200-1384_L

Plasmid

#146825

Purpose

Insect Expression of DmGW182_1200-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182_1200-1384 (gw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgRNA_Ago1_5

Plasmid

#172470

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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