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Plasmid#159574PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6-MBPExpressionInsectMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only
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pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD395
Plasmid#163102PurposeExpression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmCherry_H-NSdbd
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLO ddd64.1
Plasmid#123121PurposeE. coli expression vector for catalytically inactivated deltaproteobacteria CasXDepositorInsertCasX
UseCRISPRTagsExpressionMutationMutated amino acids in catalytic triad to alanine…PromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GWRev_Dest
Plasmid#86954PurposeGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 GW-mCit-PA
Plasmid#113449PurposeProteinA-mCitrine Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseGateway shuttle vectorTagsmCitrine-ProteinAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-short Cre-on G/W
Plasmid#86949PurposeGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAVDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-2A-rtTA-TcVA
Plasmid#24592DepositorInsertGateway(TM) cassette
UseLentiviralTagsVAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-DEST_R1R4
Plasmid#97186PurposeFor fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination VectorDepositorTypeEmpty backboneUseLentiviral; GatewayTagsV5ExpressionMammalianMutationPromoterEF1 alphaAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2(fs)-Gal11p(fs)
Plasmid#127483PurposeNegative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interactionDepositorInsertLGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationOne nucleotide inserted between lambda CI and LGF…PromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2(fs)
Plasmid#127482PurposeNegative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interactionDepositorInsertGal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationLGF2 is frame shifted by the insert of one nucleo…PromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-GW
Plasmid#113448PurposeProteinA-mCitrine Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseGateway shuttle vectorTagsProteinA-mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-actin prom-dsRed-scramble shRNA
Plasmid#67879PurposeAAV vector to express dsRed sh RNA (scramble)DepositorInsertactin prom-dsRed-scramble shRNA
UseAAV and RNAiTagsExpressionMammalianMutationPromoterchicken beta actinAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCapV
Plasmid#183028Purposethe patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28DepositorInsertpatatin-like phospholipase CapV
UseTagsNoExpressionBacterialMutationPromoterpBAD promoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBK.134
Plasmid#187219PurposeExpresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectivelyDepositorInsertA: Retron Eco1 ncRNA v35 (long a1/a2); B: Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterA: pTet*; B: pBetIAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP1363
Plasmid#119279Purposefor stabilizing ICE in the presence of rapI expressionDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT[Hygro]_GBP-3xFlag-DEST
Plasmid#157813PurposeA gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selectionDepositorInsertGFP-binding protein
UseFor drosophila cellsTags3xFlagExpressionInsectMutationPromotermetallothioneinAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term
Plasmid#182926PurposeExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.DepositorTypeEmpty backboneUseTagsAPEX2 and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only