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Plasmid#33248DepositorInsertFGFR2 (Fgfr2 Mouse)
UseTagsHis and MycExpressionMammalianMutationKpnI site introduced at stop codon of FGFR2 to be…PromoterCMVAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA
Plasmid#154776Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation102TAG 150TAA in GFP reporter, hybrid PylT with A…PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B10
Plasmid#172851PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7a-5p
Plasmid#103146PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Mito-7
Plasmid#57969PurposeLocalization: Mitochondria, Excitation: 549, Emission: 565DepositorInsertMito (COX8A Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE
Plasmid#138144PurposeExpresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caacDepositorInsertPguCas13b
UseLentiviralTagsNESExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB90 - pAGM4723_VirGN54D
Plasmid#123187PurposeGolden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in AgrobacteriaDepositorTypeEmpty backboneUseLevel 2 acceptor vector for expression in plantsTagsExpressionPlantMutationVirGN54D for increase virulencePromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trCD4o
Plasmid#203751PurposeEncodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrCD4o (CD4 Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30a-3p
Plasmid#103409PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30a-3p target (MIR30A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i1 sgRNA / hSpCas9
Plasmid#172825PurposeMammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg1(C)-SH2
Plasmid#46469DepositorInsertPLCg1(C)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 656-766PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-TNKS-2
Plasmid#154149PurposeFor making TNKS-2 RNADepositorInsertTankyrase 2 (TNKS2 Human)
UseIn vitro transcriptionTagsFLAGExpressionMutationPromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D
Plasmid#110300PurposeExpresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD1 Flag
Plasmid#74893PurposeExpress COMMD1 in mammalian cellsDepositorInsertCOMMD1 (COMMD1 Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172670PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertSDHB pegRNA and SDHB_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.5
Plasmid#111058PurposeExpresses dLight1.5 in mammalian cellsDepositorInsertdLight1.5
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK2 WT
Plasmid#12182DepositorInsertMEKK2 (Map3k2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG
Plasmid#74261PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.Y591X truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i4 sgRNA / hSpCas9
Plasmid#172828PurposeMammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC
Plasmid#140199PurposeEpisomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.DepositorInsertdSpCas9-VPR
UseCRISPR and Synthetic Biology; Expression in asper…TagsVPR (VP64-p65-Rta), NLSExpressionMutationPromoterPgpdAAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only