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Showing: 6681 - 6700 of 15009 results

  1. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  2. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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  15. LentiGuide_DKO

    Plasmid
    #183193
    Purpose
    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183193-plasmid-map-sequence-id-360099

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  16. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  17. pMOD_C2200

    Plasmid
    #91082
    Purpose
    Module C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none (will be fused to gRNA array in MODULE B)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91082-plasmid-map-sequence-id-182046

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Showing: 6681 - 6700 of 15009 results