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Plasmid#123908PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_029
Plasmid#123909PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA3 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_013
Plasmid#123867PurposeEncodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK systemDepositorInsertWPRE-Blast (no terminator)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_018
Plasmid#123733PurposeEncodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK systemDepositorInsertspdCas9(1-204)-frb-D10A-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_019
Plasmid#123734PurposeEncodes tamoxifen activated split-dCAS9 (N-Terminal) as a Type 3 part to be used in the MTK systemDepositorInsertspdCas9(1-204)-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_015
Plasmid#123678PurposeEncodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-2xHS4-LoxP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD277
Plasmid#125098PurposeExpression of SO_2193(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_2193(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR357.20
Plasmid#125108PurposeExpression of narL(1-154aa)-ydfI(148-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)154
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR322.2
Plasmid#125117PurposeExpression of uhpA(1-118aa)-ydfI(121-213aa), narL126 chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsuhpA(REC)-ydfI(DBD)126
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR359.4
Plasmid#125115PurposeExpression of narL(1-159aa)-uhpA(142-129aa) chimera under PLtetO-1, output Promoter PuhpT99DepositorInsertsnarL(REC)-uhpA(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.16
Plasmid#125083PurposeExpression of ompR(1-140aa)-ccaR(130-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)140
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD287
Plasmid#125103PurposeExpression of SO_4637(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4637(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD263
Plasmid#125094PurposeExpression of torR(1-134aa)-ccaR(127-234aa), ompR137 chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertstorR(REC)-ccaR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD266
Plasmid#125096PurposeExpression of torR(1-134aa)-yxdJ(132-229aa), ompR137 chimera under PLtetO-1, output Promoter PyxdJ181DepositorInsertstorR(REC)-yxdJ(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD281
Plasmid#125100PurposeExpression of SO_4428(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4428(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD285
Plasmid#125102PurposeExpression of SO_4623(1-124aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4623(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR276.4
Plasmid#125092PurposeExpression of ompR(1-122aa)-psdR(119-237aa) chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsompR(REC)-psdR(DBD)122
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR240
Plasmid#125090PurposeExpression of ompR(1-137aa)-psdR(134-237aa) chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsompR(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.25
Plasmid#125085PurposeExpression of ompR(1-110aa)-ccaR(107-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)110
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR322.4
Plasmid#125119PurposeExpression of uhpA(1-141aa)-ydfI(153-213aa), narL159 chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsuhpA(REC)-ydfI(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only