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Plasmid#71612PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tyrosine-154 to phenylalanine, tryptophan…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK510
Plasmid#71600PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp165>Phe mutant (AaPurE1wwf-H59N)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparagine, tryptophan-16…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGC291
Plasmid#19684DepositorInsertPhlh-12
UseGateway entry cloneTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGC450
Plasmid#19691DepositorInsertPhlh-12
UseGateway entry cloneTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGC451
Plasmid#19692DepositorInserthlh-12_coding region
UseGateway entry cloneTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu_PRC1(WT)
Plasmid#136349PurposeHuman PCR1 cDNA in Gateway pENTRDepositorInsertPRC1 (PRC1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
474 p5E-basprom
Plasmid#31158DepositorInsertbasal adenovirus E1b TATA element
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
P2r-P3 Ste3pmCherry
Plasmid#58561PurposepDONR P2r-P3 carrying the STE3 promoter mCherry fusion (reverse orientation)DepositorInsertSte3promoter-mCherry
UseGateway entryTagsExpressionMutationPromoterAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO GFP
Plasmid#69798PurposeGateway entry vector for GFPDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
R777-E011 Hs.ARHGAP35
Plasmid#70295PurposeGateway ORF clone of human ARHGAP35 [NM_004491.4] with stop codon (for native or N-terminal fusions)DepositorInsertARHGAP35 (ARHGAP35 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Plasmid#140082PurposeEntry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoterDepositorInsertpT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
UseIn vitro transcription (mrna synthesis)TagsExpressionBacterialMutationPromoterT7Available sinceOct. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E012 Hs.ARHGAP35-nostop
Plasmid#70296PurposeGateway ORF clone of human ARHGAP35 [NM_004491.4] without stop codon (for C-terminal fusions)DepositorInsertARHGAP35 (ARHGAP35 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
R777-E114 Hs.JUN-nostop
Plasmid#70398PurposeGateway ORF clone of human JUN [NM_002228.3] without stop codon (for C-terminal fusions)DepositorInsertJUN (JUN Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PTPN11 in pENTR
Plasmid#216604Purposegateway cloning donor vector containing PTPN11DepositorInsertPTPN11 (PTPN11 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Dest
Plasmid#79784PurposeExpression plasmid - BsmBI entry sitesDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterpTrcAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E041 Hs.DUSP5
Plasmid#70325PurposeGateway ORF clone of human DUSP5 [NM_004419.3] with stop codon (for native or N-terminal fusions)DepositorInsertDUSP5 (DUSP5 Human)
UseGateway entry cloneTagsExpressionMutationA220M (human SNP)PromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -