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Showing: 6581 - 6600 of 6974 results

  4. pPlacPbuCas13b-gRNA-NT

    Plasmid
    #184838
    Purpose
    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184838-plasmid-map-sequence-id-373981

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  5. pSBK.136

    Plasmid
    #187220
    Purpose
    Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectively
    Depositor
    Seth Shipman
    Article
    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:
    Insert
    Retron Eco1 ncRNA v35 (long a1/a2); Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    A: pSalTTC; B: pTet*
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187220-plasmid-map-sequence-id-368082

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  6. KTK_139

    Plasmid
    #180565
    Purpose
    Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Insert
    Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    180565-plasmid-map-sequence-id-373326

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  7. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

    Plasmid
    #186413
    Purpose
    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    lacZ (lacZ E. coli)
    Use
    Gateway destination vector
    Tags
    Nuclear localization signal
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186413-plasmid-map-sequence-id-369044

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  8. KTK_095

    Plasmid
    #180524
    Purpose
    dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    180524-plasmid-map-sequence-id-359542

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  11. pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)

    Plasmid
    #160582
    Purpose
    35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    attR:P35S:TMtb:attL
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160582-plasmid-map-sequence-id-322083

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  13. pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

    Plasmid
    #159838
    Purpose
    Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159838-plasmid-map-sequence-id-317992

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  20. MTK234_056

    Plasmid
    #123921
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    gRNA-GFPDROPOUT-hu6_20N_cr3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123921-plasmid-map-sequence-id-250076

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Showing: 6581 - 6600 of 6974 results