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Plasmid#218039PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00AZ-W
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00H3-S-FLAG-His
Plasmid#218038PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00H3-S
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00FH-2-FLAG-His
Plasmid#218037PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00FH-2
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00B0-E-FLAG-His
Plasmid#218035PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00B0-E
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIFEX-A2aGH
Plasmid#160542PurposepITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFEX1-A2aGH
Plasmid#160543PurposepYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX1-A2aGH
Plasmid#160546PurposepYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)
Plasmid#156417Purpose8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sitesDepositorInsertmurine Dll4 F2-6/F8 ETS site B/ site #2, 8X
UseZebrafish transgenesisTagsExpressionMutationPromoterE1b min pro/b-globin intronAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
Plasmid#73796PurposepRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNADepositorInsertsdCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1, pRPR1(TetO), and pTef1Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0-6H-TEV-KRAS G12D
Plasmid#159439PurposeExpresses KRAS G12D in E.coliDepositorInsertKRAS G12D (KRAS Human)
UseTags6xHISExpressionBacterialMutationPromoterT5Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/XBB.1.5-RBD
Plasmid#213038PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variantDepositorInsertpCAGGS/XBB.1.5-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUDP002
Plasmid#103872PurposeBroad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)DepositorInsertshph
Spcas9 D147Y P411T
UseCRISPRTagsNLSExpressionYeastMutationD147Y P411TPromoterArxula adeninivorans TEF1 and Ashbya gossypii (Er…Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Omicron.BA.5-RBD
Plasmid#213478PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variantDepositorInsertpCAGGS/Omicron.BA.5 RBD (S )
UseTags6 His TagExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/XBB.1.16-RBD
Plasmid#213070PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variantDepositorInsertpCAGGS/XBB.1.16-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/EG5-RBD
Plasmid#213410PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variantDepositorInsertpCAGGS/EG5-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso AdelCTD
Plasmid#137722PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)DepositorInsertBRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTruncated at amino acid 1328PromoterTight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)
Plasmid#156407PurposepCS2 CMV expression vector driving EGFP fused human KRAS4B-G12VDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseTagsEGFPExpressionMammalianMutationmutant KRAS4B-G12VPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)
Plasmid#156411PurposepCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4ADepositorInserthuman wild type KRAS4A-WT (KRAS Human)
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 763 (pCS2-EYFP-hsKRAS4B-WT)
Plasmid#156408PurposepCS2 CMV expression vector driving EYFP fused human KRAS4BDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)
Plasmid#156405PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codonDepositorInserthuman wild type KRAS4A-WT (KRAS Human)
UseGateway cloningTagsmScarlet-IExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only