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msPlekha3 (msFapp1) g1 lentiCRISPRv2-opti

Plasmid

#218648

Purpose

Knockout vector for mouse Plekha3 (Fapp1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Plekha3 (Plekha3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msPlekha8 (msFapp2) g2 lentiCRISPRv2-opti

Plasmid

#218651

Purpose

Knockout vector for mouse Plekha8 (Fapp2)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Plekha8 (Plekha8 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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PLEKHA3 (FAPP1) g2 lentiCRISPRv2-opti

Plasmid

#218657

Purpose

Knockout vector for human PLEKHA3 (FAPP1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

PLEKHA3 (PLEKHA3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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PLEKHA8 (FAPP2) g1 lentiCRISPRv2-opti

Plasmid

#218658

Purpose

Knockout vector for human PLEKHA8 (FAPP2)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

PLEKHA8 (PLEKHA8 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PLEKHA3 (FAPP1) g1 lentiCRISPRv2-opti

Plasmid

#218656

Purpose

Knockout vector for human PLEKHA3 (FAPP1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

PLEKHA3 (PLEKHA3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218637

Purpose

Proximity biotinylation control of OSBP(PH domain)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

PH domain only

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-OmpA-NHSTIIT-GG

Plasmid

#215790

Purpose

Cloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-SufI-NHSTIIT-GG

Plasmid

#215791

Purpose

Cloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC- AVITAG-CTH10STII- -GG

Plasmid

#215805

Purpose

Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNIC-HIS6-SUMO1-AVITAG-GG

Plasmid

#215808

Purpose

Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTwist-msEEA1

Plasmid

#214991

Purpose

Gateway entry vector with mouse EEA1

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (Eea1 Mouse)

Use

Gateway

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pIA035

Plasmid

#200426

Purpose

amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Insert

LacI

Use

Tags

dronPA

Expression

Bacterial

Mutation

Promoter

Ppen

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pIA030

Plasmid

#200424

Purpose

thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Insert

LacI

Use

Tags

PAmCherry

Expression

Bacterial

Mutation

Promoter

Ppen

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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nTALE+CD_for TUB_8N_1397NC

Plasmid

#193383

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For multiplex C-to-T base editing to introduce amino acid mutations in the coding sequence of AtTUBs.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG2-RPS5Ap-NLS-OleGFP

Plasmid

#193370

Purpose

nTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4-R3_HSPter-RPS5A-NLS

Plasmid

#193371

Purpose

nTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
nTALECD for_CYO1 exon1_1397NC

Plasmid

#193376

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
nTALECD for_CYO1 exon2_1397NC

Plasmid

#193377

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
nTALECD for_PKT3 exon1_1397NC

Plasmid

#193378

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtPKT3.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
nTALECD for_PKT3 exon12_1397NC

Plasmid

#193379

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtPKT3.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge