Skip to main content
Addgene
Showing: 641 - 660 of 1639 results

  1. pAAV-CAG-DIO-PPO-Venus

    Plasmid
    #139504
    Purpose
    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has Service
    AAV5
    Insert
    parapinopsin
    Use
    AAV
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139504-plasmid-map-sequence-id-276180

    Enlarge

  2. pGEM-HE-Glyco-Venus-bPAC(S27A)

    Plasmid
    #165490
    Purpose
    Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity
    Depositor
    Thomas Oertner
    Article
    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10
    Insert
    Glyco-Venus-bPAC(S27A)-Myc
    Use
    Cdna expression, xenopus oocyte
    Tags
    Myc and Venus
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165490-plasmid-map-sequence-id-330308

    Enlarge

  4. pSCM167.2-Venus-VF-hADAM10(aa1-213)

    Plasmid
    #189554
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 1-213
    Promoter
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189554-plasmid-map-sequence-id-384131

    Enlarge

  5. pSCM167-Venus-VF-hADAM10(aa220-654)

    Plasmid
    #189560
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 220-654
    Promoter
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    189560-plasmid-map-sequence-id-384135

    Enlarge

  6. pSCM167-Venus-VF-hADAM10(aa1-459)

    Plasmid
    #189559
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 1-459
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189559-plasmid-map-sequence-id-374396

    Enlarge

  10. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid
    #68345
    Purpose
    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii
    Inserts
    puromycin
    hCas9
    Use
    CRISPR; Flp/frt
    Tags
    linked to red flouresent protein gene mKateII and…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68345-plasmid-map-sequence-id-438782

    Enlarge

  14. pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

    Plasmid
    #69587
    Purpose
    Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus
    Depositor
    Galit Lahav
    Article
    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:
    Insert
    p53-L344P (TP53 Human)
    Use
    Lentiviral
    Tags
    mKate2 and split mVenus - C term
    Expression
    Mammalian
    Mutation
    p53 L344P mutation that prevents dimerization and…
    Promoter
    EF1alpha
    Available since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    69587-plasmid-map-sequence-id-135230

    Enlarge

  20. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid
    #193720
    Purpose
    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Synthetic, Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193720-plasmid-map-sequence-id-383731

    Enlarge

Showing: 641 - 660 of 1639 results