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Addgene
Showing: 641 - 660 of 1585 results
  1. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA
    Depositor
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Availability
    Academic Institutions and Nonprofits only
  2. pShuttle-CMV-F2A-T2A-Venus

    Plasmid
    #62621
    Purpose
    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tag
    Depositor
    Insert
    MCS with F2A and T2A
    Use
    Adenoviral
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid
    #127356
    Purpose
    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and mVenus
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

    Plasmid
    #105127
    Purpose
    Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein
    Inserts
    Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    cryaa (crystallin) and fabp10a
    Availability
    Academic Institutions and Nonprofits only
Showing: 641 - 660 of 1585 results