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Plasmid#111392PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsEYFP and MycExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC
Plasmid#114376Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
UseAAVTagsEYFP, Kv2.1C, and TlcnCExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1534 pscAAV mU6 shRNA(PKCd) CMV-IE Nuc-EYFP
Plasmid#135562PurposeAn AAV vector expressing shRNA vs rat PKCd and a nuclear EYFP reporterDepositorInsertsshRNA (mouse PKCd)
Nuc-EYFP
UseAAV and RNAiTags3xNLSExpressionMammalianMutationPromoterCMV-IE and mU6Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP
Plasmid#135563PurposeAn AAV vector expressing scrambled shRNA and a nuclear EYFP reporterDepositorInsertsshRNA (scrambled)
Nuc-EYFP
UseAAV and RNAiTags3xNLSExpressionMammalianMutationPromoterCMV-IE and mU6Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Plasmid#20298PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activationDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9Insertchannelrhodopsin-2
UseAAVTagsEYFPExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC
Plasmid#114377Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targetingDepositorInsertGtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
UseAAVTagsEYFP and Kv2.1C-linker-TlcnCExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65454PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMutationH134R variantPromoterhSyn1Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65429PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMutationH134R variantPromoterhSyn1Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)
Plasmid#190755PurposeExpression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.DepositorUseTagsmCherry2 and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP13276: pAAV-AiE0779m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3276)
Plasmid#214842PurposeExpression of ChR2(H134R)-EYFP in striatal direct pathway medium spiny neurons (D1 MSNs)InsertChR2(H134R)-EYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755)
Plasmid#214846PurposeExpression of ChR2(H134R)-EYFP in striatal cholinergic neuronsHas ServiceAAV PHP.eBInsertChR2(H134R)-EYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP14870 - pAAV-AiE0452h_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN4870)
Plasmid#224186PurposeAiE0452h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSNsInsertChR2(H134R)-EYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP14823: pAAV-AiE0441h_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN4823)
Plasmid#214860PurposeExpression of ChR2(H134R)-EYFP in striatal medium spiny neuronsInsertChR2(H134R)-EYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP13278: pAAV-AiE0779m_3xC2-minBG-ChR2(CRC)-EYFP-WPRE3-BGHpA (Alias: CN3278)
Plasmid#214843PurposeExpression of ChR2(L132C, H134R, T159C)-EYFP in striatal direct pathway medium spiny neurons (D1 MSNs)InsertChR2(L132C, H134R, T159C)-EYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP12577 - pAAV-AiE0140h_3xC2-minBG-ChR2(CRC)-EYFP-WPRE3-BGHpA (Alias: CN2577)
Plasmid#224166PurposeAiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+ neuronsInsertChR2(CRC)-EYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-eYFP (AAV5)
Viral Prep#117382-AAV5PurposeReady-to-use AAV5 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-eYFP (AAV2)
Viral Prep#117382-AAV2PurposeReady-to-use AAV2 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-eYFP (AAV9)
Viral Prep#117382-AAV9PurposeReady-to-use AAV9 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-eYFP (AAV8)
Viral Prep#117382-AAV8PurposeReady-to-use AAV8 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-eYFP (AAV PHP.eB)
Viral Prep#117382-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only