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Plasmid#113880Purposep3E entry vector for Three Multisite GatewayDepositorInserthe1a (he1.1 Zebrafish)
UseZebrafish danio expressionTagsECFPExpressionMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BG4-NLS-mCer
Plasmid#211448Purposemammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequenceDepositorInsertAnti-DNA G-quadruplex single chain antibody BG4
UseTagsNLS-mCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-AktAR2
Plasmid#125195PurposeSB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporterDepositorInsertAktAR2
UseTransposonTags6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Puro_LPAR_AKAR-WT
Plasmid#125206PurposeSB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporterDepositorInsertLPAR_AKAR-WT
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmRFPmars
Plasmid#26252DepositorInsertmRFPmars
UseTags6xHisExpressionBacterialMutationKpnI restriction site removedPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-Lyn-AktAR2-EV
Plasmid#125199PurposeSB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporterDepositorInsertLyn-AktAR2-EV
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-AktAR2-D
Plasmid#125196PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporterDepositorInsertAktAR2-D
UseTransposonTags6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionRD
Plasmid#172924PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRD
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionRE
Plasmid#172926PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Puro_LPAR_AKAR-mut
Plasmid#125207PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporterDepositorInsertLPAR_AKAR-mut
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionKE
Plasmid#172925PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorKE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_crGE
Plasmid#172933PurposeCrowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-1.0 L
Plasmid#18721DepositorInsertdTomato ; mCerulean ; EYFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-1.1 M
Plasmid#18722DepositorInsertKusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Thy1-Brainbow-2.1 R
Plasmid#18727DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Thy1-Brainbow-1.1 M
Plasmid#18726DepositorInsertKusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-1.0 H
Plasmid#18720DepositorInserttdimer2 ; M-mEYFP ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ionRD
Plasmid#172931PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.DepositorInsertIonicStrengthSensorRD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only