Addgene

We narrowed to 2,130 results for: CO

Showing: 641 - 660 of 2130 results

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pDSHA-ARF4-Myc

Plasmid

#67350

Purpose

Mammalian expression of hArf4 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)_No_FLAG_ATP1A1-G7

Plasmid

#173203

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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CAGhCBFA2T2

Plasmid

#84363

Purpose

FLAG-HA tagged human CBFA2T2

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

CBFA2T2 (CBFA2T2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pM-ErbB4CTF

Plasmid

#17797

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment only, amino acids 676-1…

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
ATP1A1_G8_Dual_sgRNA

Plasmid

#178104

Purpose

Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G8 sgRNA + user-specified sgRNA

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-UL87-2xStrep

Plasmid

#138434

Purpose

Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87 (UL87 HCMV (HHV-5))

Use

Tags

Strep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARFRP1-GFP

Plasmid

#67408

Purpose

Mammalian expression of hArfrp with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL11-GFP

Plasmid

#67407

Purpose

Mammalian expression of hArl11 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

bp 442 T to C; aa C148R

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pminiT-Halo-MED1 donor

Plasmid

#197868

Purpose

Donor plasmid used for building Halo-MED1 knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

CRSP210 (Med1 Mouse)

Use

Mouse Targeting

Tags

Halotag

Expression

Mutation

Promoter

NA

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARF1-GFP

Plasmid

#67390

Purpose

Mammalian expression of hArf1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV6-SMAC-HA-eGFP

Plasmid

#67492

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Use

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARF4-V5-His6

Plasmid

#67434

Purpose

Mammalian expression of hArf4 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4-GFP

Plasmid

#67398

Purpose

Mammalian expression of hArl4 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

bp 105 A to T; silent mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARF1-V5-His6

Plasmid

#67432

Purpose

Mammalian expression of hArf1 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARFRP1

Plasmid

#67471

Purpose

Mammalian expression of hArfrp with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA

Plasmid

#178107

Purpose

Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G8 + MTOR nick exon-53 sgRNAs

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X-ORF24-NTD

Plasmid

#138465

Purpose

Expresses MBP-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

MBP

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARF4-GFP

Plasmid

#67392

Purpose

Mammalian expression of hArf4 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pGEX4T1-ORF24-NTD

Plasmid

#138464

Purpose

Expresses GST-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA

Plasmid

#178110

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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