1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pBZS-YP_001497877

   Plasmid

   #198366

   Purpose

   Express YP_001497877 in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   YP_001497877 (B681L )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBZS-YP_001498157

   Plasmid

   #198369

   Purpose

   Express YP_001498157 in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   YP_001498157 (C075L )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNL-5.1

   Plasmid

   #190589

   Purpose

   Lentiviral vector-mediated delivery of artificial exons for protein tagging in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Bialkowska et al BMC Genomics. 2005 Sep 4;6:113. doi: 10.

   Inserts

   NeoR transgene
   artificial exon encoding BSD and EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Cellular and SV40

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2+-dCas9-olEzh2(deltaSET)

   Plasmid

   #180217

   Purpose

   Ezh2 (delta SET domain) fused to dCas9 for H3K27me3 epigenome editing control.

   Depositor

   Hiroyuki Takeda

   Article

   Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

   Insert

   enhancer of zeste 2 polycomb repressive complex 2 subunit (deltaSET)
   

   Use

   Tags

   FLAG and dCas9

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBL-2

   Plasmid

   #162769

   Purpose

   Minimal backbone with AmpR

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Molecular cloning

   Tags

   Expression

   Mutation

   Promoter

   no

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEDJ-22

   Plasmid

   #140904

   Purpose

   MarkerFree plasmid for integration of pADH1-PCP-Mxi1 into site X-4

   Depositor

   Michael Jensen

   Article

   Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

   Insert

   PCP-Mxi1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   ADH1

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET19b_STS:P4S

   Plasmid

   #139797

   Purpose

   expression of biosynthetic enzyme STS in fusion with CC segment P4S

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Insert

   STS:P4S
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET19b_4CL:STS (DF)

   Plasmid

   #139791

   Purpose

   direct fusion of biosynthetic enzymes 4CL and STS

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Insert

   direct fusion of 4CL and STS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFH132

   Plasmid

   #136547

   Purpose

   Level1 ZmUBIp::FnCas12a (human codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::FnCas12a (human codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFH133

    Plasmid

    #136548

    Purpose

    Level1 ZmUBIp::LbCas12a (human codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::LbCas12a (human codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFH25

    Plasmid

    #131764

    Purpose

    Level0 SpCas9, wheat codon optimized (version 3)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    SpCas9, wheat codon optimized, version 3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFH109

    Plasmid

    #128197

    Purpose

    Level1 ZmUBIp::nScCas9 (wheat codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::nScCas9 (wheat codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFH39

    Plasmid

    #126013

    Purpose

    Level0 Rice OsU3 Pol III promoter with transcription Start-A

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Level0 Rice OsU3 Pol III promoter with transcription Start-A
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFH60

    Plasmid

    #128186

    Purpose

    Level1 ZmUBIp::FnCas12a (rice codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::FnCas12a (rice codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFH61

    Plasmid

    #128187

    Purpose

    Level1 ZmUBIp::LbCas12a (rice codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::LbCas12a (rice codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFH77

    Plasmid

    #125816

    Purpose

    Level0 enCas9-PolI3M-TBD, wheat codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    enCas9-PolI3M-TBD, wheat codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFH91

    Plasmid

    #125819

    Purpose

    Level0 nScCas9 (nickase), wheat codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    nScCas9 (nickase), wheat codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC57-CmYLCV

    Plasmid

    #205245

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-Neq2X7

    Plasmid

    #182366

    Purpose

    Expresses Neq2X7 DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X7 DNA polymerase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHREAC

    Plasmid

    #134908

    Purpose

    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites.

    Depositor

    George Lomonossoff

    Article

    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    double 35S

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge