Skip to main content
Addgene
Showing: 6561 - 6580 of 22131 results

  1. VRER variant

    Plasmid
    #138561
    Purpose
    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRER
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138561-plasmid-map-sequence-id-274783

    Enlarge

  5. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

    Enlarge

  7. pEGFP-PH-SWAP70(R223E, R224E)

    Plasmid
    #84583
    Purpose
    Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding
    Depositor
    Geert van den Bogaart
    Article
    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do
    Insert
    SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin-homology domain, residues 209-306, mut…
    Promoter
    CMV
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Cynthia Collins
    Article
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53440-plasmid-map-sequence-id-83235

    Enlarge

  11. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD and PLtetO
    Available since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

    Enlarge

  12. CoV2-N-P13L

    Plasmid
    #177946
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (P13L) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    P13L
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177946-plasmid-map-sequence-id-348197

    Enlarge

  13. CoV2-N-P80R

    Plasmid
    #177947
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (P80R) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    P80R
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177947-plasmid-map-sequence-id-348196

    Enlarge

  14. CoV2-N-S235F

    Plasmid
    #177956
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (S235F) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S235F
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177956-plasmid-map-sequence-id-348185

    Enlarge

  16. EF1a_MYC deltaHLH_P2A_Hygro_Barcode

    Plasmid
    #120508
    Purpose
    Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaHLH (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of helix-loop-helix motif
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120508-plasmid-map-sequence-id-234930

    Enlarge

Showing: 6561 - 6580 of 22131 results