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Plasmid#178219PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HA.S and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HA.HSV_mCherry-NLS
Plasmid#178216PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HA.HSV and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.VSVg_mCherry-NLS
Plasmid#178214PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.VSVg and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.S_mCherry-NLS
Plasmid#178213PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.S and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.HSV_mCherry-NLS
Plasmid#178210PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.HSV and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Ctdnep1_D67E-2xFlag
Plasmid#196519PurposeExpression of Ctdnep1_D67E-2xFlagDepositorInsertCtdnep1_D67E-2xFlag (CTDNEP1 Human)
UseTags2xFlagExpressionMammalianMutationD67E mutation (phosphastase dead)PromoterCMV promoter; T7 promoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+CeGFP-Ctdnep1WTsiR
Plasmid#196517PurposeExpression of Ctdnep1WT-GFPDepositorInsertCtdnep1WTsiR (CTDNEP1 Human)
UseTagseGFPExpressionMammalianMutationSilent mutations for siRNA resistancePromoterCMV promoter; T7 promoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterMyl7 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet
Plasmid#176535PurposeNon-protein coding locus components from CRISPR-associated IscBDepositorInsertnon-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha1-390
Plasmid#62521PurposeC-terminal aa391-1374 of Drosha is deletedDepositorInserthuman Drosha with aa 391-1374 deleted (DROSHA Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 391-1374PromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-DVL2deltaDIX
Plasmid#114276PurposeTET-inducible expression of DVL2 protein with a deletion of its DIX-domainDepositorInsertDVL2 (Dvl2 Mouse)
UseLentiviralTagsFLAGExpressionMutationdeletion of DIX domainPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
VP16-ER beta (short)
Plasmid#11353DepositorInsertER beta short (ESR2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Klf4
Plasmid#136613PurposeDox-inducible lentiviral vector expressing mouse Klf4DepositorInsertKlf4 (Klf4 Mouse)
UseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_01
Plasmid#158102Purpose5'UTR stem-loop luciferase reporter: 0kcal/molDepositorInsert5'UTR synthetic stem-loop 0kcal/mol
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP
Plasmid#55642PurposesCre-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAV; Scre-dependentTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX
Plasmid#163571PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-NG-nCas9-RBMX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3-K51A
Plasmid#155265PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationLysine 51 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3-R70A
Plasmid#155264PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationArginine 70 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-APPL1-R146A/K152A/R154A
Plasmid#59767PurposeExpresses APPL1 Endosomal Localization MutantDepositorInsertAPPL1 (APPL1 Human)
UseTagsEGFPExpressionMammalianMutationR146A/K152A/R154APromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only