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Plasmid#222346PurposepegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA2 with trimmed attP
Plasmid#222347PurposepegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG_GPHN.FingR-eGFP-CCR5TC
Plasmid#46296DepositorInsertGPHN.FingR-eGFP-CCR5TC (GPHN Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
EPS15-GFP-His
Plasmid#170860PurposeMammalian expression of EPS15-GFP-His.DepositorInsertEpidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-8
Plasmid#208786PurposeInducibly expresses human Caspase 8 fused to light-activatable Cry2DepositorInsertCaspase 8 (CASP8 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationCARD domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN251
Plasmid#188471PurposeLentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)DepositorInsertenCas12a-HF (gift from Dr. Keith Joung)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3nls
Plasmid#155366PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertdCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseCRISPRTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133APromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorInsertFYVE-Hrs x 2 (PIKFYVE Human)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro
Plasmid#74947PurposeDosage control and tracing of reprogramming episomesDepositorInsertOct3/4 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCXLE-hUL + mTAGBFP2
Plasmid#74944PurposeDosage control and tracing of reprogramming episomesDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pFUGW-Pol2-ffLuc2-eGFP
Plasmid#71394PurposeLentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoterDepositorInsertPol2-ffLuc2-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterPol2 (mouse RNA polymerase II gene promtoer)Available sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab7 WT
Plasmid#12605DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEJS578_DD-dSpyCas9-mCherry-APEX2
Plasmid#108570PurposeExpression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factorsDepositorInsertdSpyCas9
UseCRISPR and LentiviralTagsAPEX2, Destabilization domains, Flag, and mCherry…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA
Plasmid#205317PurposeAll-in-one AAV vector expressing ISYmu1 TnpB and its reRNADepositorInserthumanized ISYmu1 TnpB
UseAAVTags3 x HAExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre
Plasmid#84561PurposepSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loopsDepositorInsertFLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
UseLentiviralTagsFlag, Suntag, oxBFP, AID (auxin-inducible degron)ExpressionMutationPromoterUbCAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
7M8
Plasmid#64839PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.DepositorInsert7M8 cap
UseAAVTagsExpressionMutation7mer insertion in the AAV2 capPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
IMPT-10314:GABBR2
Plasmid#157851PurposeHA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expressionDepositorInsertGABBR2 (GABBR2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only