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Plasmid#61206PurposeFluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoterDepositorInsertAtPDLP1
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
p201B Cas9
Plasmid#59177PurposeCas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
BAR
UseCRISPRTagsExpressionMutationPromoter2x35SAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-ro1GFP
Plasmid#82369PurposeExpression of redox sensitive GFP in mammalian cellsDepositorInsertro1GFP
UseTagsExpressionMammalianMutationC48S/F64L/T65S/S147C/Q204CPromoterCMVAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMU-TPr
Plasmid#61189PurposeFluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoterDepositorInsertAtTIP1-1
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
V245 pCEP-4HA B56alpha
Plasmid#14532DepositorInsertPPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
UseTags4 HAExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-P2A-HSA
Plasmid#121936PurposeCRISPR-Sirius plasmidDepositorInsertdCas9-P2A-HSA
UseCRISPR and LentiviralTagsHSAExpressionMammalianMutationPromoterCMV-TOAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
5HT6-mCherry-G-GECO1.0
Plasmid#47500PurposeFluorescent reporter for Ca2+ inside primary cilia with mCherry referenceDepositorInsertG-GECO1.0
UseTags5HT6 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMU-NUCr
Plasmid#61168PurposeFluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoterDepositorInsertNLS-mCherry-GUS
UseTagsExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-FRB
Plasmid#183403PurposeExpresses nuclear-localized LgBiT-FRBDepositorInsertLgBiT-FRB
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-R-iGluSnFR1
Plasmid#107335PurposeRed fluorescent glutamate sensor based on cpmApple. Membrane-displayedDepositorInsertR-iGluSnFR1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTOX8
Plasmid#127453PurposeEncodes MqsR toxin and tsPurple chromoprotein. CAM-RDepositorInsertsmqsR toxin
tsPurple chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterapFAB46 and pRhaAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSelAct-KO
Plasmid#174374PurposePlasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatibleDepositorTypeEmpty backboneUseVector for 2-step knockout by homologous recombin…TagsExpressionMutationPromoterN/AAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATM N-terminal sgRNA
Plasmid#207089PurposepX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.DepositorInsertATCATTAAGTACTAGACTCA
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-GFP
Plasmid#127888PurposeProvides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP taggingDepositorInsertPuro-GFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMU-MTUBr
Plasmid#61196PurposeFluorescent reporter for microtubules mCherry-MAP4-MBD expressed under AtUBQ10 promoterDepositorInsertMAP-MBD
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
YEp181-CUP1-His-Ubi
Plasmid#99539PurposeYeast episomal vector for expression of His-tagged ubiquitin; LEU2 markerDepositorInsertUBI3(1-76) (RPL40A Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTagJr-MBP
Plasmid#105628PurposePlasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigaseDepositorInsertSnoopTagJr-MBP
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-mito-tagRFP
Plasmid#185599PurposeLentiviral construct for expression of tagRFP-T targeted to mitochondriaDepositorInsertmito-tagRFP-T
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
MKbpAII
Plasmid#91689PurposeMMTV-LTR promoter/enhancer with rabbit beta-globin intron and bovine growth hormone poly-A sequence to enhance transgene expressionDepositorInsertMMTV LTR cloned into KbpA vector
UseTagsExpressionMammalianMutationPromoterMMTV LTRAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp R4-4 mutant
Plasmid#173491PurposeExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.DepositorInsertSfp-R44
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only