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Plasmid#99325PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ127B
Plasmid#99883PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoterDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10
Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
UseTagsHAExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mus-Bnip3_deltaExon3
Plasmid#100793PurposeMammalian expression of Bnip3_deltaExon3 splice variantDepositorInsertmouse-Bnip3_deltaExon3 (Bnip3 Mouse)
UseTagsHAExpressionMammalianMutationExon3 deletionPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhE_AW-pHG165
Plasmid#90062PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE_AW
UseTagsExpressionBacterialMutationI48V/Q55A/Q60RPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhS-pHG165a
Plasmid#90040PurposeConstitutive expression of a chimeric LacI:GalS transcription repressorDepositorInsertLLhS
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhF-pHG165a
Plasmid#90041PurposeConstitutive expression of a chimeric LacI:FruR transcription repressorDepositorInsertLLhF
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhT-pHG165a
Plasmid#90042PurposeConstitutive expression of a chimeric LacI:TreR transcription repressorDepositorInsertLLhT
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhR-pHG165a
Plasmid#90043PurposeConstitutive expression of a chimeric LacI:RbsR transcription repressorDepositorInsertLLhR
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhA-pHG165a
Plasmid#90044PurposeConstitutive expression of a chimeric LacI:AscG transcription repressorDepositorInsertLLhA
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhE-pHG165
Plasmid#90045PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhC-pHG165a
Plasmid#90046PurposeConstitutive expression of a chimeric LacI:CytR transcription repressorDepositorInsertLLhC
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala13
Plasmid#87174PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proDAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)
Plasmid#86922PurposeTo express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.DepositorInsertUBL4A (UBL4A Human)
UseTagsFLAG and GFPExpressionMammalianMutationResidues from 90-157 is deletedPromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala11
Plasmid#87172PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proAAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala12
Plasmid#87173PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proCAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
const #7-RFP
Plasmid#81081Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23109Available sinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pconst #6-RFP
Plasmid#81080Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23117Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWHI2-HYG
Plasmid#80589PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoterDepositorInsertWHI2 (WHI2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterWHI2 native promoterAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only