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mCherry-DFCP1
Plasmid#86746PurposeN-terminal mCherry-tagged protein expression in mammalian cellsDepositorInserthuman DFCP1 (ZFYVE1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-ShadowY-kCAAX
Plasmid#115564Purposedark ITRACK acceptor construct for dual color imagingDepositorInsertShadowY with KRas membrane targeting domain
UseTagsShadowYExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29R
Plasmid#17602PurposeMammalian expression of HA tagged ubiquitin K29RDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29R; V5 deletedPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shBRCA1 #1
Plasmid#44594DepositorInsertBRCA1 (Brca1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shBRCA1 #2
Plasmid#44595DepositorInsertBRCA1 (Brca1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
FU-tetO-Gateway
Plasmid#43914DepositorTypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…Tags6xHis and V5ExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAD-LyseR
Plasmid#99244PurposeFor autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHKMC1: Empty sgRNA for Cloning
Plasmid#67720PurposeEmpty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterPU6Available sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku70
Plasmid#46957PurposePlasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.DepositorInsertKu70 (XRCC6 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
VPS13A^mCherry
Plasmid#118758PurposeVPS13A internally tagged with mCherry at residue 1372.DepositorInsertVPS13A (VPS13A Human)
UseTagsmCherry inserted at position 1372ExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-mCherry-HPos
Plasmid#87159PurposeFluorescent marker of lipid droplets (LDs)DepositorInsertHPos
UseTagsmCherryExpressionMammalianMutationPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-Myc-gate-pGK-HYG
Plasmid#107395PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV Fcgamma RIIa IRES neo
Plasmid#14948DepositorInsertFcgamma RIIa (FCGR2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationSRDX (X3) repressor fusion; NLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DU422gp160
Plasmid#100926PurposeMammalian expression plasmid for Env from the DU422.1 HIV-1 isolateDepositorInsertHIV-1 (DU422.1) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-ZYG11A
Plasmid#110551Purposeexpresses a FLAG-tagged human ZYG11A protein in mamalian cellsDepositorInsertZYG11A (ZYG11A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEP4-B41gp160
Plasmid#123247PurposeMammalian expression plasmid for Env from the B41 HIV-1 isolateDepositorInsertHIV-1 (B41) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only