-
Plasmid#126654PurposeInducible expression of Draper-Ala11-GFP mutant in S2 cellsDepositorInsertDraper cytoplasmi Tyr residues mutated to Ala
UseTagsExpressionInsectMutationCytoplasmic Tyr residues to AlaPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-delta ITAM-GFP
Plasmid#126655PurposeInducible expression of Draper-delta ITAM-GFP mutant in S2 cellsDepositorInsertDraper with ITAM Tyr residues mutated to Ala
UseTagsExpressionInsectMutationCytoplasmic ITAM Tyr residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT FRB-Draper INT-GFP
Plasmid#126665PurposeInducible expression of a synthetic FRB-Draper receptor in S2 cellsDepositorInsertFRB-Draper INT domain
UseTagsExpressionInsectMutationK986N- see depositor comments belowPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper delta ITAM
Plasmid#126671PurposeExpression of His10-Draper delta ITAM mutant for purification from E. coliDepositorInsertDraper cytoplasmic ITAM residues mutated to Phe
UseTagsExpressionBacterialMutationCytoplasmic ITAM residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-GFP
Plasmid#66083PurposeG protein alpha-s internally tagged with EGFP and EE epitopeDepositorInsertG-alpha-s-EE-EGFP (Gnas Rat)
UseTagsEGFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-mCherry
Plasmid#66968PurposeG protein alpha-s internally tagged with mCherry and EE epitopeDepositorInsertG-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha- s…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-CFP
Plasmid#55793PurposeG protein alpha-s internally tagged with ECFP and EE epitopeDepositorInsertG-alpha-s-EE-ECFP (Gnas Rat)
UseTagsECFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationHis was substituted for Asn164 in ECFP (Clontech)…PromoterCMVAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
CelTOS-COMP-blac-flag-his
Plasmid#111014PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertcell traversal protein for ookinetes and sporozoites (CelTOS) (PF3D7_1216600 )
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-mCerulean
Plasmid#66084PurposeG protein alpha-s internally tagged with mCerulean and EE epitopeDepositorInsertG-alpha-s-EE-mCerulean (Gnas Aequorea victoria, Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHD-dsRed-attP_dCrk_flankingHRs
Plasmid#131141PurposeContains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).DepositorUseCRISPR and Cre/Lox; Homology directed repair temp…TagsExpressionMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A-stop-mut-miR-375-mutA-E
Plasmid#53714PurposeLuciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutationsDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseTagsExpressionMutationMutation on the stop codon of firefly luciferase …PromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-gate-FLAG-IRES-GFP
Plasmid#107398PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-NRAS(G12V)
Plasmid#35632DepositorInsertN-Ras(G12V) (NRAS Human)
UseLentiviralTagsHAExpressionMammalianMutationGlycine 12 to ValinePromoterPGKAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec633
Plasmid#130873PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationL477V, L640Y, I777K, W814NPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcDNA3
Plasmid#18964DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3-EGFP-Rac1-T17N
Plasmid#12982DepositorInsertRac1 DN (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1
Plasmid#17743DepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
myc-Raptor
Plasmid#1859DepositorInsertRaptor (RPTOR Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
3X ERE TATA luc
Plasmid#11354PurposeLuciferase reporter containing three copies of vitellogenin Estrogen Response ElementDepositorInsertERE
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only