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AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (AAV8)

Viral Prep

#130909-AAV8

Purpose

Ready-to-use AAV8 particles produced from AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (#130909). In addition to the viral particles, you will also receive purified AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. CAG-driven, Flp-dependent expression of ChrimsonR-tdTomato. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Promoter

CAG

Tags

tdTomato (Flp-dependent)

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
GFP-C2gamma

Plasmid

#21215

Depositor

Tobias Meyer

Article

Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Insert

GFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

The C2-domain from PKCgamma (aa170-260) plus flan…

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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Trip-GFP-hOCLN

Plasmid

#32789

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only
pAF0033 Ef1a-Pa01-2a-EGFP

Plasmid

#193460

Purpose

Expression of Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CanlonicSF

Plasmid

#178342

Purpose

To explore lactate dynamics in intact systems

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

CanlonicSF

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDRM98 mKate2 Blast

Plasmid

#174720

Purpose

Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

mKate2
BSD

Use

Lentiviral

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Mutation

Promoter

MND and MND (off mKate-P2A)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3550

Plasmid

#145026

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDH2-WT

Plasmid

#87926

Purpose

Expresses IDH2 (wild-type) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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MAC_N_GATA4

Plasmid

#167758

Purpose

Expresses GATA4 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

GATA4 (GATA4 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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8 pCOLI_A_GST

Plasmid

#160150

Purpose

N-term GST 3C cleavable Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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SCORE

Plasmid

#40232

Depositor

Wolfhard Almers

Article

An et al Science. 2004 Nov 5;306(5698):1042-6.

Insert

Snap25b (SNAP25 Human)

Use

Tags

CFP and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-tLuc-mCherry

Plasmid

#107553

Purpose

tLuc-mCherry in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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Ef1a-DIO-BiPOLES-mCerulean

Plasmid

#154949

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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tdTomato-Mito-7

Plasmid

#58115

Purpose

Localization: Mitochondria, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Insert

Mito (COX8A Human)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/TO_TOMM20_HaloTag9

Plasmid

#169335

Purpose

Expression of HaloTag9 at the mitochondrial surface of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag9 (TOMM20 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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AICSDP-24:MYH10-mEGFP

Plasmid

#87428

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

MYH10 Homology Arms with mEGFP-linker (MYH10 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

May 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCyPet-His

Plasmid

#14030

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

Insert

CyPet

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE8e

Plasmid

#181751

Purpose

MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE8e

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRA

Plasmid

#131035

Purpose

Expresses OT-I TCRA

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Insert

TCRA (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr

Plasmid

#19325

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Punc-122::GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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