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Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flamindo2
Plasmid#73938PurposeExpresses biosensor for cAMP in mammalian cellsDepositorInsertFlamindo2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
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hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393
Plasmid#216382PurposeBaculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11DepositorInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTags6xHis-3C-splitGFP11ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRNA2-(A)128
Plasmid#174006PurposeUsed to generate stable mRNA for heterologous expression experiments (includes polyA tail)DepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
UseTags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:minP-GFP
Plasmid#109036PurposeMPRA v3 minimal promoter with GFP donorDepositorInsertemGFP
UseTagsExpressionMammalianMutationPromoterminPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA/Myc TCF4
Plasmid#16512DepositorInsertTCF4 (TCF7L2 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:∆luc:∆xbaI
Plasmid#109035PurposeMPRA v3 empty backboneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoternoneAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS3799-gRNA-Csy4-template
Plasmid#182827PurposeSpCas9 gRNA-Csy4 templateDepositorInsertSpCas9 gRNA scaffold + Csy4 sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLA2G15-6xHis WT
Plasmid#213603PurposeExpresses PLA2G15-6xHis in mammalian cellsDepositorInsertPhospholipase A2 Group XV (PLA2G15 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWS5321-CRISPRai-Markerless
Plasmid#182828PurposeSaccharomyces cerevisiae inducible CRISPRai vector - markerlessDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYMA3_SidK_GFP
Plasmid#184276PurposeLegionella pneumophila SidK (1-278) - GFPDepositorInsertSidK (1-278) (sidK Legionella pneumophila)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2-Nuc
Plasmid#14893PurposeMammalian expression of EBFP2 blue fluorescent protein with NLSDepositorInsertpEBFP2-Nuc
UseTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pWS5396-CRISPRai-KanR
Plasmid#182835PurposeSaccharomyces cerevisiae inducible CRISPRai vector - KanR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5398-CRISPRai-HygR
Plasmid#182837PurposeSaccharomyces cerevisiae inducible CRISPRai vector - HygR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#82400PurposeGateway-compatible zebrafish elavl3 promoterDepositorInsertelavl3 promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLH-nmsgRNA1.1
Plasmid#64115PurposeVector for expression of Nm sgRNA1.1 in mammalian cellsDepositorInsertNm sgRNA1.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWS5390-CRISPRai-URA3
Plasmid#182829PurposeSaccharomyces cerevisiae inducible CRISPRai vector - URA3 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only