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Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc
Plasmid#113862PurposeTriple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MECP2
Plasmid#47806PurposeExpresses methyl-CpG binding protein 2 (Rett Syndrome)DepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
tetO-SOX9
Plasmid#170703Purposedoxycycline-inducible overexpression of SOX9DepositorInsertSOX9 (SOX9 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-MEF2C
Plasmid#46031DepositorInsertMEF2C (MEF2C Human)
UseLentiviralTagsHis and V5ExpressionMammalianMutationPromotertetOAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-hEPO
Plasmid#50436Purposeexpress human erythropoietin using lentiviral delivery systemDepositorInsertEPO (EPO Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
tetO-SMAD6
Plasmid#170700Purposedoxycycline-inducible overexpression of SMAD6DepositorInsertSMAD6 (SMAD6 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest-303
Plasmid#159678PurposeGateway destination vector for mammalian protein expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RRV
Plasmid#188543PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editingDepositorInsertLbCas12a-RRV
UseCRISPR; Gateway compatible lbcas12a-rrv entry clo…TagsNLSExpressionPlantMutationG146R, D156R, R182VPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-PBX1
Plasmid#170699Purposedoxycycline-inducible overexpression of PBX1DepositorInsertPBX1 (PBX1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk12_Nterm_FlagHa
Plasmid#127177PurposeN-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vectorDepositorInsertN-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UseEntry vector with transgene for gateway cloningTagsFlag- HA- Tandem EpitopesExpressionMutationPromoterNoneAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRARE-MBP-DEST
Plasmid#84650PurposeGateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.DepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromotertacAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO-MYOCD
Plasmid#170696Purposedoxycycline-inducible overexpression of MYOCDDepositorInsertMYOCD (MYOCD Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RVQ
Plasmid#200187PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editingDepositorInsertLbCas12a-RVQ
UseCRISPR; Gateway compatible lbcas12a-rv entry cloneTagsNLSExpressionPlantMutationG146R, R182V, E795QPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ223 (dAsCpf1-SRDX)
Plasmid#86209PurposeDeactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertdAsCpf1
UseGateway compatible cpf1 vectorsTagsNLS and SRDX repressor and NLSExpressionPlantMutationD908APromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-GW-HA-RINT1
Plasmid#39841DepositorInsertRINT1 (RINT1 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2
Plasmid#206261PurposeENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.DepositorTypeEmpty backboneUseMultimate/gateway entr 2TagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-SMARCD3
Plasmid#170701Purposedoxycycline-inducible overexpression of SMARCD3DepositorInsertSMARCD3 (SMARCD3 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLgw EcoDam-V5-RFC1
Plasmid#59209PurposeMammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloningDepositorTypeEmpty backboneUseLentiviral; DamidTagsDam and V5ExpressionMammalianMutationPromoterHeat Shock Minimal PromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only