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Showing: 6401 - 6420 of 150386 results

  2. pQCXIB (w297-1)

    Plasmid
    #22800
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 17, 2009
    Availability
    Academic Institutions and Nonprofits only
    22800-plasmid-map-sequence-id-10440

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  6. pcDNA3.1-ME-Flag

    Plasmid
    #101860
    Purpose
    Mds1-Evi-1 flag tagged for transfection into mammalian cells
    Depositor
    Archibald Perkins
    Insert
    Mds1-Evi-1 (Mecom Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101860-plasmid-map-sequence-id-211085

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  14. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

    Plasmid
    #208957
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208957-plasmid-map-sequence-id-415746

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  15. MRGPRF-Tango

    Plasmid
    #66437
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MRGPRF (MRGPRF Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  20. AURKB

    Plasmid
    #39119
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AURKB (AURKB Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 6401 - 6420 of 150386 results