Addgene

We narrowed to 22,131 results for: Nov

Showing: 6381 - 6400 of 22131 results

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pHR-CMV-TetO2_HA-BirA

Plasmid

#113896

Purpose

Mammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p45)

Plasmid

#135947

Purpose

Expresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCAGGS mSynCAM1

Plasmid

#115496

Purpose

expression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expression

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm1 (Cadm1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta-actin

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only
TIA1 RRM-mCherry-iLIDx6

Plasmid

#103782

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-mCherry-iLIDx6

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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CoV2-N-R203K

Plasmid

#177951

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203K) (N )

Use

Tags

Expression

Mammalian

Mutation

R203K

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_LHX3_P2A_Hygro_Barcode

Plasmid

#120456

Purpose

Barcoded lentiviral vector to express LHX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LHX3 (LHX3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDmAct5C::Cas9-2A-Neo

Plasmid

#176679

Purpose

Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)

Use

Crispr

Tags

Expression

Insect

Mutation

Drosophila codon optimized

Promoter

D. melanogaster Act5C

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-2delta

Plasmid

#124146

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Marius Sudol, Megan Finch-Edmondson

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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LLP788_Traptavidin-EZH2-FL-CO

Plasmid

#211781

Purpose

AviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-EZH2

Use

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.8

Plasmid

#102917

Purpose

expresses ABE7.8 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.8

Use

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJH4504

Plasmid

#191702

Purpose

Pnmr-1 long::TeTx::wCherry unc-54 3' UTR C.elegans AVA/other neurons expression of TeTx RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Tetanus Toxin Light Chain

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pnmr-1 long

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10B-HA

Plasmid

#209216

Purpose

Mammalian expression of ATP10B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP10B (ATP10B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-L-R279E

Plasmid

#182286

Purpose

Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-flag Mst2 (KR)

Plasmid

#27372

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mst2 (STK3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

K56R (catalytically inactive)

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only
FASTHDR-Cterm-mRuby3-Zeo

Plasmid

#167206

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2-CreLite

Plasmid

#131783

Purpose

Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC, PIF6CreN, and mTagBFP2

Use

Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Ubi

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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