Addgene

We narrowed to 1,780 results for: ipp

Showing: 621 - 640 of 1780 results

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pFA6a-GST-His3MX6

Plasmid

#41604

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

Plasmid

#52549

Purpose

transgenic construct for expression of GFP and Flpo

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP
Flpo recombinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120391

Purpose

pAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Type

Empty backbone

Use

AAV

Tags

mCherry.3XFLAG

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti GFP-AMOT p130 Y242/287A

Plasmid

#32829

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Use

Lentiviral

Tags

GFP-AMOT

Expression

Mammalian

Mutation

Y242/287A

Promoter

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only
pCA-T-intron-G

Plasmid

#36887

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-GFP

Plasmid

#41615

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP(S65T), TRP1 reverse orientation, and yeast AD…

Expression

Mutation

Promoter

Available since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron-T

Plasmid

#36885

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pIW1

Plasmid

#52530

Purpose

serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Twin-STREP-tag

Use

Blunt cloning kit

Tags

Expression

Mutation

Promoter

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pJP102

Plasmid

#176480

Purpose

Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Inserts

actin > NatR
EF1 > mScarlet

Use

Capsaspora owczarzaki expression

Tags

Expression

Mutation

Promoter

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJP103

Plasmid

#176481

Purpose

Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Inserts

actin > HygR
EF1 > mScarlet

Use

Capsaspora owczarzaki expression

Tags

Expression

Mutation

Promoter

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCA-ATG-intron-tdT3Myc

Plasmid

#36883

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tdTomato-3Myc

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

beta-globin intron containing a loxP site inserte…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pHR'-ROMK1-CITE-GFP

Plasmid

#21322

Depositor

Jay Nadeau

Article

Nadeau et al J Neurophysiol. 2000 Aug . 84(2):1062-75

Insert

ROMK1 (Kcnj1 Rat)

Use

Lentiviral

Tags

GFP (bicistronic with CITE/IRES)

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S61A

Plasmid

#33087

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Tags

Flag

Expression

Mammalian

Mutation

S61A

Promoter

CMV

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only
MTK2_024

Plasmid

#123716

Purpose

Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pPGK1c

Use

Tags

Expression

Mammalian

Mutation

Crippled Kozac sequence

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCB-GCN

Plasmid

#127476

Purpose

coding sequence for synthetic zinc finger ZF-BCB-GCN

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Expression

Mutation

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRB20

Plasmid

#52559

Purpose

serves as PCR-template to generate HR donors for C-terminal YFP-tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

eYFP and Blasticigin resistance cassette

Use

Blunt cloning kit

Tags

Expression

Mutation

Promoter

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-SETin-FLAG-HA

Plasmid

#24999

Depositor

Judy Lieberman

Article

Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Insert

SET (SET Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations introduced to prevent siRNA targ…

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pF(UG) hSyn SYP-SELF

Plasmid

#158770

Purpose

Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptophysin 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Hoxc4

Plasmid

#45550

Depositor

Jeremy Dasen

Article

Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

Insert

Hoxc4 (Hoxc4 Mouse)

Use

Tags

Expression

Mutation

Promoter

CMV / b-actin

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-ybbR-HIS-CBM-CohI

Plasmid

#58709

Purpose

Probing molecule to perform single molecule force spectroscopy experiments

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

CBM-CohI

Use

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

changed Alanine 2 to Cysteine in CBM domain

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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