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Plasmid#196495PurposeCRISPR tool to target astrocytesDepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pgfaABC1D-mCherry-CAAX
Plasmid#196488PurposeTo over express mCherry-CAAX under gfaABC1D promoter in astrocytesDepositorInsertmCherry-CAAX
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap-KO
Plasmid#196489PurposeTo KO Gfap in mouse astrocytesDepositorInsertgRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTvGPI(EP)-blast-mNG
Plasmid#221051PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTvGPI(VSG)-blast-mNG
Plasmid#221050PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mStayGold[E138D]
Plasmid#221049PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmStayGold[E138D]
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3
Plasmid#218639PurposeGateway shuttling vector for cloning C-terminal NLRP3 tags (contains no stop codon).DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Roquin isoform A (HK63)
Plasmid#206469PurposeRoquin expression in Schneider cells for imagingDepositorInsertRoquin (roq Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-mEGFP-CAF1 isoform A (HK50)
Plasmid#206437PurposeCAF1 expression in Schneider cells for imagingDepositorInsertCAF1 (Pop2 Fly)
UseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-mEGFP-CAF40 isoform A (HK51)
Plasmid#206438PurposeCAf40 expression in Schneider cells for imagingDepositorInsertCAf40 (Rcd-1 Fly)
UseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-mEGFP-human FRB (HK168)
Plasmid#206443PurposeFRB expression in Schneider cells for imagingDepositorInsertFRB (MTOR Human)
UseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKDC.100
Plasmid#222224PurposeAcceptor plasmid for yeast retron-Eco1 ncRNA variant libraries.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKDC.154
Plasmid#222228PurposeAcceptor plasmid for human retron-Eco1 variants.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
SH4-FRB-EBFP2
Plasmid#222418PurposeTo express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertSH4
UseTagsFRB-HA-EBFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-4
Plasmid#221490PurposeNKX2-4 lentiviral overexpressionDepositorInsertNKX2-4 (NKX2-4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-4
Plasmid#221478PurposeGateway cloning of NKX2-4 overexpressionDepositorInsertNKX2-4 (NKX2-4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
F17-pAc5.1 EGFP Vasa 1-200
Plasmid#206484Purposevasa 1-200 expression for Schneider cell imagingDepositorInsertVasa 1-200
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
msPlekha8 (msFapp2) g1 lentiCRISPRv2-opti
Plasmid#218650PurposeKnockout vector for mouse Plekha8 (Fapp2)DepositorInsertPlekha8 (Plekha8 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLEKHA8 (FAPP2) g2 lentiCRISPRv2-opti
Plasmid#218659PurposeKnockout vector for human PLEKHA8 (FAPP2)DepositorInsertPLEKHA8 (PLEKHA8 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
msPlekha3 (msFapp1) g2 lentiCRISPRv2-opti
Plasmid#218649PurposeKnockout vector for mouse Plekha3 (Fapp1)DepositorInsertPlekha3 (Plekha3 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only