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Plasmid#186393PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
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pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1
Plasmid#124651PurposeSoma-localized ChrimsonR for dense expression in excitatory neuronsDepositorHas ServiceAAV9InsertChrimsonR
UseAAVTagsmScarletExpressionMammalianMutationPromoterCamKIIaAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-BBS3/Arl6 [N302/10R]
Plasmid#188211PurposeMammalian Expression Plasmid of anti-BBS3/Arl6 (Human). Derived from hybridoma N302/10DepositorInsertanti-BBS3/Arl6 (Homo sapiens) recombinant mouse monoclonal antibody (ARL6 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAH49 - [PVQp | mScarlet | tbb-2 UTR]
Plasmid#200325PurposemScarlet BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVQp | wrmscarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003755, nlp-17Available sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-oScarlet-WPRE
Plasmid#183518PurposeOptogeneticsDepositorInsertChRmine-oScarlet
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAH61 - [ASELp | wrmScarlet | tbb-2 UTR]
Plasmid#200335PurposemScarlet BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[ASELp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00001534, gcy-7Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-V12
Plasmid#125136PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).DepositorInsertmNeon/mScarletP2A Rac1-V12
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarlet. 12th AA in Rac1 ch…PromoterCAGAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-oScarlet
Plasmid#137138PurposeIntersectional viral expression of oScarlet in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-N17
Plasmid#125137PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).DepositorInsertmNeon/mScarletP2A Rac1-N17
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarlet. 17th AA in Rac1 ch…PromoterCAGAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)
Plasmid#156411PurposepCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4ADepositorInserthuman wild type KRAS4A-WT (KRAS Human)
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mU6-GFPGO2-IRES-mScarletI
Plasmid#136896PurposeLentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.DepositorInsertGFPGO2-IRES-mScarletI
UseLentiviralTags2xNLS on GFP, 3xNLS on mScarletExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mU6-GFPGO-IRES-mScarletI
Plasmid#136895PurposeLentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.DepositorInsertGFPGO-IRES-mScarletI
UseLentiviralTags3xNLS on mScarletExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSAH85 - [AWCp | wrmScarlet | tbb-2 UTR]
Plasmid#200359PurposemScarlet BioPart for AWC neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWCp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00012770, srt-47Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH
Plasmid#203841Purposenon-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expressionDepositorInsertKir2.1 (Kcnj2 Mouse)
UseAAVTags2A-mScarlet-KASHExpressionMutationG144A, Y145A, G146APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH51 - [DDp | wrmScarlet | tbb-2 UTR]
Plasmid#200327PurposemScarlet BioPart for DD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[DDp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00007304, ttr-39Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet
Plasmid#130709PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarletDepositorInsertNTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
H2B-mScarlet- hMeikin(1-332)-mNeonGreen
Plasmid#174714PurposeStable expression of fluorescent Separase cleavage sensor with human MeikinDepositorInsertMeikin
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation1-332PromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet P2A Rac1-wt
Plasmid#125135PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged WT Rac1.DepositorInsertmNeon/mScarletP2A-Rac1
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterCAGAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-P2A-mScarlet (MNW006)
Plasmid#174136PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
RhoA sensor (LSS-SGFP2-Scarlet-I)
Plasmid#112947PurposeRhoA FRET biosensorDepositorInsertRhoA sensor
UseTagsLSS-SGFP2 and mScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only