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Plasmid#110227Purposeintegration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoterDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only
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px330 Gatad2a sgRNA (for deletion)
Plasmid#110814PurposeExpressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell linesDepositorInsertmGataD2a (Gatad2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330 Gatad2a sgRNA (for flox targeting)
Plasmid#110815PurposeUsed with pNTK Gatad2a flox allele targeting construct in mouse cellsDepositorInsertmGataD2a (Gatad2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. adolescentis mRNA toehold switch sensor
Plasmid#110710PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertB. adolescentis species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-Sfxn2 (CG6812)
Plasmid#110643PurposeRetroviral expression of Flag-tagged Drosophila CG6812 in mammalian cellsDepositorInsertCG6812 (CG6812 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-Sfxn1-3 (CG11739)
Plasmid#110642PurposeRetroviral expression of Flag-tagged Drosophila CG11739 in mammalian cellsDepositorInsertCG11739 (CG11739 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW8
Plasmid#110222PurposeCre-expression plasmid for cassette exchangeDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW8_yEGFP
Plasmid#110229PurposeCre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent proteinDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRSP-COMP-blac-flag-his
Plasmid#110964PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertthrombospondin related sporozoite protein (TRSP) (PF3D7_0104000 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_0910300-COMP-blac-flag-his
Plasmid#110959PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein, unknown function (PF3D7_0910300 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CCp1-COMP-blac-flag-his
Plasmid#110999PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertLCCL domain-containing protein (CCp1) (PF3D7_1475500 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CCp2-COMP-blac-flag-his
Plasmid#110998PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertLCCL domain-containing protein (CCp2) (PF3D7_1455800 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PSOP7-COMP-blac-flag-his
Plasmid#110996PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertsecreted ookinete protein, putative (PSOP7) (PF3D7_1340000 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHT1-COMP-blac-flag-his
Plasmid#110995PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertchitinase (CHT1) (PF3D7_1252200 )
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
P28-COMP-blac-flag-his
Plasmid#110990PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertookinete surface protein P28 (PF3D7_1030900 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_0505700-COMP-blac-flag-his
Plasmid#110988PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium membrane protein (PF3D7_0505700 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1123500-COMP-blac-flag-his
Plasmid#110987PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein (PF3D7_1123500 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_0526900-COMP-blac-flag-his
Plasmid#110985PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInserttransmembrane emp24 domain-containing protein, putative (PF3D7_0526900 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV500
Plasmid#110722PurposeLentiviral system for reduced ectopic expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only