We narrowed to 15,824 results for: LEA

1. Cre-p2a-tdTomato

   Plasmid

   #176837

   Purpose

   Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Cre-P2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1-ADGRL3-Flag

   Plasmid

   #201218

   Purpose

   pCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal Flag

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

   Plasmid

   #214733

   Purpose

   Expresses mCherry protein in infected and cre positive cells

   Depositor

   Félix Leroy

   Article

   de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

   Insert

   anti-Crh shRNA (scrambled) / mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-vCre

   Plasmid

   #55638

   Purpose

   EF1a-driven expression of VCre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Insert

   vCre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-SYP1-miniSOG-citrine

   Plasmid

   #50971

   Purpose

   AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Insert

   Synaptophysin-miniSOG-citrine (Syp Rat)
   

   Use

   AAV

   Tags

   citrine and miniSOG

   Expression

   Mutation

   Promoter

   human synapsin promoter

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-ABE7.9

   Plasmid

   #102918

   Purpose

   expresses ABE7.9 in mammalian cells

   Depositor

   David Liu

   Article

   Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

   Insert

   ABE7.9
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFBD-Akt13C(S473D)

   Plasmid

   #86577

   Purpose

   Expresses cleavable human full-length Akt1(S473D) phosphomimetic

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130; S473D phos…

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY002 (pFnCpf1_min)

   Plasmid

   #69975

   Purpose

   Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 locus without Cas genes nd artificial promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYFP-ErbB2

    Plasmid

    #66948

    Purpose

    Mammalian expression of rat ErbB2 tagged with YFP

    Depositor

    Bo van_Deurs, Mads Lerdrup

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    (See depositor comment below on mutations A24T an…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-FLAG-Grb10-WT

    Plasmid

    #74501

    Purpose

    Overexpression of Grb10 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al Mol Oncol. 2013 Jun;7(3):402-18. doi: 10

    Insert

    Grb10 (GRB10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L358S, V522A, and V524L

    Promoter

    CMV

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pBJG1 T7-8xHis-OGT K842M

    Plasmid

    #154272

    Purpose

    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    Lysine 842 changed to Methionine- bacterial codon…

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_Anc689-NG-nCas9

    Plasmid

    #163527

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-DNM2-R465W

    Plasmid

    #155262

    Purpose

    Mammalian expression of rat DNM2 fused to HA

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Arginine 465 to Tryptophan

    Promoter

    Available since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

    Plasmid

    #122084

    Purpose

    Express M.EcoGII fused to TRF1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-TERF1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of TERF1

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBS1449 SED1-Lenti

    Plasmid

    #180824

    Purpose

    SED1 biosensor for stable integration and expression in mammalian cells

    Depositor

    Rajat Rohatgi

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 osmosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSI-Target-AID-NG

    Plasmid

    #119861

    Purpose

    Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-B-evoSpCas9-NLS-6xHis

    Plasmid

    #207390

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9-NRCH-SsAPOBEC3B

    Plasmid

    #198550

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-HisMBP-SED1

    Plasmid

    #178191

    Purpose

    SED1 biosensor for expression in Escherichia coli

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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