We narrowed to 15,981 results for: LEA

1. pCDNA3.1-ADGRL3-Flag

   Plasmid

   #201218

   Purpose

   pCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal Flag

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFBD-Akt13C(S473D)

   Plasmid

   #86577

   Purpose

   Expresses cleavable human full-length Akt1(S473D) phosphomimetic

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130; S473D phos…

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-5X-3-SARS-CoV-2-3CL

   Plasmid

   #168457

   Purpose

   Bacterial codon optimized expression vector for SARS-CoV-2 3CL protease.

   Depositor

   David Ho, Sho Iketani, Alejandro Chavez

   Article

   Iketani et al Nat Commun. 2021 Apr 1;12(1):2016. doi:

   Insert

   SARS-CoV-2 3CL protease (ORF1ab Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   March 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pHBS1449 SED1-Lenti

   Plasmid

   #180824

   Purpose

   SED1 biosensor for stable integration and expression in mammalian cells

   Depositor

   Rajat Rohatgi

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 osmosensor
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-HisMBP-SED1

   Plasmid

   #178191

   Purpose

   SED1 biosensor for expression in Escherichia coli

   Depositor

   Cesar Cuevas-Velazquez, Jose Dinneny

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 biosensor
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-7 aSyn C141

   Plasmid

   #108866

   Purpose

   Expresses human WT alpha synuclein with a C-terminal cysteine

   Depositor

   Gabriele Kaminski Schierle

   Article

   Stephens et al bioRxiv 2022.10.04.510646

   Insert

   alpha synuclein (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   C141 mutation to allow C-terminal maleimide dye l…

   Promoter

   T7

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1_VNp-mNeongreen-StefinA

   Plasmid

   #182419

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-stefin (CSTA Human)
   

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-Grb10-WT

   Plasmid

   #74501

   Purpose

   Overexpression of Grb10 in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al Mol Oncol. 2013 Jun;7(3):402-18. doi: 10

   Insert

   Grb10 (GRB10 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L358S, V522A, and V524L

   Promoter

   CMV

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

   Plasmid

   #122084

   Purpose

   Express M.EcoGII fused to TRF1 with detabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII-V5-TERF1
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of TERF1

   Expression

   Bacterial and Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TCR012H

    Plasmid

    #233454

    Purpose

    Expresses ncHLAp-reactive T cell receptor (TCR012H)

    Depositor

    William Freed-Pastor

    Article

    Ely et al Science. 2025. May 8; 388(6747)

    Insert

    TCR012H
    

    Use

    Lentiviral

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3XHalo_GFP_TRF1#1

    Plasmid

    #164644

    Purpose

    tag TRF1 with GFP and Halo

    Depositor

    Huaiying Zhang, Michael Lampson

    Article

    Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYFP-ErbB2

    Plasmid

    #66948

    Purpose

    Mammalian expression of rat ErbB2 tagged with YFP

    Depositor

    Bo van_Deurs, Mads Lerdrup

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    (See depositor comment below on mutations A24T an…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCMV_Anc689-NG-nCas9

    Plasmid

    #163527

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBactin-Tubg1-mEGFP

    Plasmid

    #196870

    Purpose

    Expression of the gamma-tubulin fused to enhanced (E)-GFP

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Tubg1-mEGFP (Tubg1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid

    #127356

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9-NRCH-SsAPOBEC3B

    Plasmid

    #198550

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB_OTmut

    Plasmid

    #185388

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSP2133

    Plasmid

    #72249

    Purpose

    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y450A, N497A, R661A, Q695A, and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-ABE6.3

    Plasmid

    #102916

    Purpose

    expresses ABE6.3 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE6.3
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSP1101

    Plasmid

    #65773

    Purpose

    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, and T1337R mutations in C…

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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