We narrowed to 16,633 results for: LEA

1. mADAM10 deltaMP in pQCXIP cFLAG

   Plasmid

   #19138

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM10 (Adam10 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   delta 56-457

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1

   Plasmid

   #176537

   Purpose

   Non-protein coding locus components from KraIscB-1

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   non-coding locus components from KraIscB-1 locus
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNAintron-CHIKV-nsp1-c3xFLAG

   Plasmid

   #215694

   Purpose

   Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV nsp1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNAintron-CHIKV-nsp2-c3xFLAG

   Plasmid

   #215695

   Purpose

   Produces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV nsp2
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNAintron-CHIKV-nsp4-c3xFLAG

   Plasmid

   #215697

   Purpose

   Produces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV nsp4
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCMV_APOBEC1-UdgX-nCas9

   Plasmid

   #163531

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY005 (pcDNA3.1-hLb3Cpf1)

   Plasmid

   #69977

   Purpose

   Expresses humanized Lb3Cpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLb3Cpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

   Plasmid

   #102986

   Purpose

   AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   membrane-targeted SNAP protein after FLP-mediated recombination
   Nuclear-targeted eGFP after FLP-mediated recombination
   

   Use

   AAV; Adeno associated viral vector

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_VNp6-StefinA

   Plasmid

   #182404

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-stefin (CSTA Human)
   

   Tags

   Vesicle Nucleating peptide 6 (VNp6)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU387

    Plasmid

    #178981

    Purpose

    pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    CRISPR array from Candidatus Scalindua brodae
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRGEB-VirD2-Cas9

    Plasmid

    #139803

    Purpose

    Expressing VirD-Cas9 fusion under ubiquitin promoter

    Depositor

    Magdy Mahfouz

    Article

    Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

    Insert

    VirD2-Cas9
    

    Tags

    VirD2-Cas9

    Expression

    Plant

    Promoter

    Ubiquitin

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. phFluc-WT

    Plasmid

    #177714

    Purpose

    Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

    Depositor

    Erik Böttger

    Article

    Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

    Insert

    hFluc WT
    

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. HOXA4 (murine) HA-tag pET

    Plasmid

    #8489

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXA4 (Hoxa4 Mouse)
    

    Tags

    HA and T7

    Expression

    Bacterial

    Mutation

    Thr-2 changed to Ala-2 to form better ribosomal b…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pGEXNKI-his-GST-3C-LIC

    Plasmid

    #108709

    Purpose

    Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    GST-6xHis-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF Doc2alpha WT GFP

    Plasmid

    #128819

    Purpose

    To visualize Doc2alpha WT in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. evoCas9

    Plasmid

    #138558

    Purpose

    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    evoCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. EYFP-NLS-actin-K68/284R

    Plasmid

    #60616

    Purpose

    Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

    Depositor

    Primal de Lanerolle

    Article

    Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    K68R, 284R

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLV-EF1a-Tmx4-V5

    Plasmid

    #120236

    Purpose

    Expresses V5-tagged Tmx4 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Tmx4 (Tmx4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK354

    Plasmid

    #129698

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120010799
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-miniSOG-VAMP2-citrine

    Plasmid

    #50969

    Purpose

    AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    miniSOG-VAMP2-citrine (Vamp2 Arabdopsis thaliana, Mouse)
    

    Use

    AAV

    Tags

    citrine and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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