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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,995 results for: LEA

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Showing: 6341 - 6360 of 16995 results

  2. xCas9

    Plasmid
    #138565
    Purpose
    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    xCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138565-plasmid-map-sequence-id-274797

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  6. CT691

    Plasmid
    #233660
    Purpose
    BLADE (two-input, four-output decoder); Cre excises lox sites to express GFP, Flp excises FRT sites to express iRFP, and the presence of both recombinases is designed to express mRuby.
    Depositor
    Wilson Wong
    Article
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Insert
    BLADE
    Expression
    Mammalian
    Mutation
    None
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233660-plasmid-map-sequence-id-464957

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  8. BB44_pmc.DonorR5.TS

    Plasmid
    #199223
    Purpose
    Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
    Use
    Gene targeting donor plasmid
    Expression
    Mammalian
    Promoter
    Human PGK1 gene promoter
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199223-plasmid-map-sequence-id-391672

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  10. pTM

    Plasmid
    #162783
    Purpose
    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Type
    Empty backbone
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO Elongation factor 1A
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162783-plasmid-map-sequence-id-333802

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  13. SiC-V2-Cas9G7

    Plasmid
    #133043
    Purpose
    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.
    Depositor
    Sriram Neelamegham
    Article
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    Tet repressor
    Use
    Lentiviral
    Tags
    Cerulean
    Available Since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    133043-plasmid-map-sequence-id-264424

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Showing: 6341 - 6360 of 16995 results