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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,056 results for: LEA

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Showing: 6341 - 6360 of 14056 results

  4. Ec dnaN-pHK/P

    Plasmid
    #237239
    Purpose
    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    Tags
    his-10+PKA kinase motif+PreScission Protease clea…
    Expression
    Bacterial
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    237239-plasmid-map-sequence-id-469256

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  5. pPgiCas13b-gRNA-NT

    Plasmid
    #184835
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Publication
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184835-plasmid-map-sequence-id-377028

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  6. pPbuCas13b-gRNA-1

    Plasmid
    #184567
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Publication
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184567-plasmid-map-sequence-id-374002

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  8. pDRFLIP42

    Plasmid
    #65518
    Purpose
    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Publication
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Tags
    6His-Cit-attR1 and attR2-mCer-cMyc
    Expression
    Yeast
    Promoter
    pPMA1
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65518-plasmid-map-sequence-id-111428

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  9. pTC151

    Plasmid
    #70015
    Purpose
    TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral Replicon
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (TALENs) + Donor + GVR
    Use
    TALEN
    Expression
    Plant
    Available since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    70015-plasmid-map-sequence-id-124553

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  10. pCVL TAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid
    #50420
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Publication
    Boissel et al Nucleic Acids Res. 2013 Nov 26.
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50420-plasmid-map-sequence-id-75338

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  11. eSrtA-MT3

    Plasmid
    #200305
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Publication
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT3 (MT3 Human)
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200305-plasmid-map-sequence-id-394866

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  12. pCDNA5/FRT-ChemoC

    Plasmid
    #193809
    Purpose
    CMV overexpression of ChemoC in mammalian cell lines
    Depositor
    Julien Hiblot
    Publication
    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ChemoC
    Tags
    10xHis
    Expression
    Mammalian
    Mutation
    mCerulean3:T225R; HT7: E143R, E147R, L271E
    Promoter
    CMV
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193809-plasmid-map-sequence-id-381724

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  13. pPgiCas13b-gRNA-1

    Plasmid
    #184834
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Publication
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184834-plasmid-map-sequence-id-377030

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  19. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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Showing: 6341 - 6360 of 14056 results