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pET28a MISTIC

Plasmid

#85999

Purpose

bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

Depositor

Wolfram Antonin

Publication

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

N/A

Tags

HIS6 and MISTIC

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pmCherry-Akt1(WT)

Plasmid

#86631

Purpose

Expresses mCherry-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmCherry-Akt1(WT)

Plasmid

#86631

Purpose

Expresses mCherry-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGWB411

Plasmid

#74805

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB411

Plasmid

#74805

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-hPAX3 (#395)

Plasmid

#78335

Purpose

Overexpression of human PAX3 ORF with a N-terminal FLAG tag

Depositor

Matthew Alexander, Louis Kunkel

Insert

PAX3 (PAX3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
FLAG-hPAX3 (#395)

Plasmid

#78335

Purpose

Overexpression of human PAX3 ORF with a N-terminal FLAG tag

Depositor

Matthew Alexander, Louis Kunkel

Insert

PAX3 (PAX3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Publication

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Publication

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-FLAG-AKAP-Lbc

Plasmid

#67571

Purpose

Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells

Depositor

Graeme Carnegie

Publication

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

A-kinase anchoring protein Lbc (AKAP13 Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

R2604G in AKAP

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pEGFP-FLAG-AKAP-Lbc

Plasmid

#67571

Purpose

Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells

Depositor

Graeme Carnegie

Publication

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

A-kinase anchoring protein Lbc (AKAP13 Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

R2604G in AKAP

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pHYRSF53

Plasmid

#64696

Purpose

His-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Publication

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, HisTag, and NpuDnaE-N102

Expression

Bacterial

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pHYRSF53

Plasmid

#64696

Purpose

His-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Publication

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, HisTag, and NpuDnaE-N102

Expression

Bacterial

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Ii-Str_SBP-mCherry-Ecadherin

Plasmid

#65289

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ii-Str_SBP-mCherry-Ecadherin

Plasmid

#65289

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Publication

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Publication

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pFETCH_CREB1

Plasmid

#64938

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human CREB1

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

CREB1 Homology Arms (CREB1 Human)

Tags

3X Flag P2A Neo

Expression

Mammalian

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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