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pEMS1697

Plasmid

#29292

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple225 (TBR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1704

Plasmid

#29294

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple232 (TNNT1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1722

Plasmid

#29298

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple250 (VIP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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EH1

Bacterial Strain

#102804

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
bMS.346

Bacterial Strain

#220588

Purpose

Increase editing efficiency of retron and oligo recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only
Mouse Chromatin Regulator Library

Pooled Library

#200011

Purpose

CRISPR KO library targeting a comprehensive set of genes with annotated function as chromatin regulators.

Depositor

Bradley Bernstein, Gabriel Griffin, Robert Manguso

Article

Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only
Barcode Library 1 (BCL1)

Pooled Library

#179852

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code CCAA.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
Barcode Library 0 (BCL0)

Pooled Library

#179851

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code ACGT.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
pLEX_307-SOD1WT

Plasmid

#83444

Purpose

Mammalian expression of SOD1WT

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133insTT

Plasmid

#83446

Purpose

Mammalian expression of SOD1E133insTT.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E133insTT (after GA)

Promoter

EF-1α

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133Δ

Plasmid

#83445

Purpose

Mammalian expression of SOD1E133Δ.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E133Δ

Promoter

EF-1α

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSOD1E133K-AcGFP1

Plasmid

#83442

Purpose

Expresses SOD1E133K in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

E133K

Promoter

CMV

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5K85R

Plasmid

#98694

Purpose

Gateway cloning of human PRDX5-K85R

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K85R

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5K83R

Plasmid

#98693

Purpose

Gateway cloning of human PRDX5-K83R

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K83R

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5E80K

Plasmid

#98692

Purpose

Gateway cloning of human PRDX5-E80K

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E80K

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133K

Plasmid

#83447

Purpose

Mammalian expression of SOD1E133K

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E133K

Promoter

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSOD1E133insTT-AcGFP1

Plasmid

#83441

Purpose

Expresses SOD1E133insTT in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

E133insTT

Promoter

CMV

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSOD1E133Δ-AcGFP1

Plasmid

#83419

Purpose

Expresses SOD1E133Δ in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

E133 deletion

Promoter

CMV

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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C321.ΔA.M9adapted

Bacterial Strain

#98568

Purpose

Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Bacterial Resistance

Ampicillin

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
JS200 strain

Bacterial Strain

#11794

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Bacterial Resistance

None

Available since

June 20, 2007

Availability

Academic Institutions and Nonprofits only