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pENTR221-IL6.Pro

Plasmid

#79491

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Il6 Promoter (IL6 Synthetic, Human)

Use

Gateway Cloning

Promoter

IL6 Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pME-3xNLS-mTagBFP2-Cre (JDW 918)

Plasmid

#224517

Purpose

A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xNLS-mTagBFP2-Cre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A26

Plasmid

#132259

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A26 (SLC25A26 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC38A9

Plasmid

#132070

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A9 (SLC38A9 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-EF1A-LivePAR(Y107A)-Hygro

Plasmid

#176073

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR (Y107A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Point mutation to convert Try107 to Ala

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A4

Plasmid

#132102

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A4 (SLC2A4 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pME-GcAMP5

Plasmid

#126569

Purpose

Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

GcAMP5

Use

Gateway Cloning

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221-EF1.Pro

Plasmid

#79488

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

EF1 Promoter (EEF1A1 Synthetic, Human)

Use

Gateway Cloning

Promoter

EF1 Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pME Actin-Vhh sfGFP (JDW 1215)

Plasmid

#224495

Purpose

Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A18

Plasmid

#132003

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A18 (SLC25A18 Human)

Expression

Mammalian

Available Since

Oct. 24, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEN_TT 3xFLAG ID2

Plasmid

#98390

Purpose

Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Gateway Cloning

Tags

3xFLAG

Promoter

TRE tight promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A31

Plasmid

#132036

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A31 (SLC22A31 Human)

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35B1

Plasmid

#132099

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35B1 (SLC35B1 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

Plasmid

#224490

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Golgi-mTagBFP-P2A-H2A-iRFP

Use

Gateway Cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC19A3

Plasmid

#132045

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC19A3 (SLC19A3 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR1A mKate2-hu_ncOGT WT

Plasmid

#154282

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway Cloning

Tags

2x FLAG and mKate2

Mutation

WT OGT

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC28A3

Plasmid

#132151

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC28A3 (SLC28A3 Human)

Expression

Mammalian

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35A3

Plasmid

#132135

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A3 (SLC35A3 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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p2R3a-3xVenusYFP-OcsT

Plasmid

#71271

Purpose

Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

3 times VenusYFP

Use

Gateway Cloning

Tags

octaline synthase terminator

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ERF

Plasmid

#98626

Purpose

Gateway entry TOPO TA cloning vector ERF

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ERF (Erf Mouse)

Use

Topo ta cloning vector

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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