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pENTR223-BRLF1(Rta)

Plasmid

#195824

Purpose

Gateway Entry Clone of Epstein-Barr virus BRLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BRLF1 (BRLF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Article

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR223-BRLF1(Rta)

Plasmid

#195824

Purpose

Gateway Entry Clone of Epstein-Barr virus BRLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BRLF1 (BRLF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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RBD-Fc

Plasmid

#164221

Purpose

Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

Spike RBD (S SARS-CoV)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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RBD-Fc

Plasmid

#164221

Purpose

Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

Spike RBD (S SARS-CoV)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RRV

Plasmid

#188543

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RRV

Use

CRISPR; Gateway compatible lbcas12a-rrv entry clo…

Tags

NLS

Expression

Plant

Mutation

G146R, D156R, R182V

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RRV

Plasmid

#188543

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RRV

Use

CRISPR; Gateway compatible lbcas12a-rrv entry clo…

Tags

NLS

Expression

Plant

Mutation

G146R, D156R, R182V

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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NEK7_HUMAN_D0

Plasmid

#79735

Purpose

This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK7 (NEK7 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
NEK7_HUMAN_D0

Plasmid

#79735

Purpose

This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK7 (NEK7 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pENTR223-BZLF1(Zta)

Plasmid

#195787

Purpose

Gateway Entry Clone of Epstein-Barr virus BZLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BZLF1 (BZLF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BZLF1(Zta)

Plasmid

#195787

Purpose

Gateway Entry Clone of Epstein-Barr virus BZLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BZLF1 (BZLF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-p5-b2m-HLA-A0101

Plasmid

#196512

Purpose

Express single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a CMV peptide, b2m and HLA A0101

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-p5-b2m-HLA-A0101

Plasmid

#196512

Purpose

Express single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a CMV peptide, b2m and HLA A0101

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-miniTurbo-L2

Plasmid

#127352

Purpose

gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-miniTurbo-L2

Plasmid

#127352

Purpose

gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMi[3xP3::mCherry-SV40, Plodia-FibL::mBaojin-P10]

Plasmid

#240410

Purpose

For Minos transgenesis in insect, expresses the 3xP3::mCherry eye/glia transgenesis, and the mBaoJin in Posterior Silk Glands (under the control of the Fibroin-L promoter from Plodia interpunctella)

Depositor

Arnaud Martin

Article

Shodja et al PeerJ. 2025 Nov 12;13:e20249. doi: 10.77

Inserts

3xP3::mCherry_SV40terminator
FibL::mBaojin_P10terminator (Fib-l Synthetic)

Expression

Insect

Promoter

3xP3 and Fibroin-L proximal promoter (Plodia inte…

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMi[3xP3::mCherry-SV40, Plodia-FibL::mBaojin-P10]

Plasmid

#240410

Purpose

For Minos transgenesis in insect, expresses the 3xP3::mCherry eye/glia transgenesis, and the mBaoJin in Posterior Silk Glands (under the control of the Fibroin-L promoter from Plodia interpunctella)

Depositor

Arnaud Martin

Article

Shodja et al PeerJ. 2025 Nov 12;13:e20249. doi: 10.77

Inserts

3xP3::mCherry_SV40terminator
FibL::mBaojin_P10terminator (Fib-l Synthetic)

Expression

Insect

Promoter

3xP3 and Fibroin-L proximal promoter (Plodia inte…

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

Plasmid

#127362

Purpose

gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

Plasmid

#127362

Purpose

gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-5

Plasmid

#228965

Purpose

For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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