We narrowed to 17,450 results for: try
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Plasmid#105837PurposeExpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 186PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HaloTag7
Plasmid#167266PurposeOverexpression of HaloTag7 in E. coliDepositorInsertHaloTag7
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-stop
Plasmid#113745PurposeContains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.DepositorInsertstop cassette
UseCRISPR; Gateway entry vectorMutationAdded 3 stop codons to the original sequence; see…Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-ReaChR-citrine
Plasmid#169045PurposeMiddle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insertDepositorInsertReaChR-citrine
UseGateway entry cloning vectorTagscitrineAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
Plasmid#113864PurposeSingle pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.DepositorInsertsPTCH1 (hedgehog dependent promoter)
mTurquoise2
UseMule gateway entry vectorExpressionMammalianAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-2PH-PLCd
Plasmid#71283PurposeMultisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-deltaDepositorInsert2PH-PLC delta (Human)
UseGateway multisite middle entry cloneAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-dead-mCherry
Plasmid#179683PurposeExpression of base editor in mammalian cells and for cell sortingDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
ExpressionMammalianMutationE1347AAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-Myc
Plasmid#177717PurposeER protein alpha-1-antitrypsin (WT), myc taggedDepositorAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
gCOTS-pyl
Plasmid#92048PurposeThis is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.DepositorInsertsPyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseCyanobacteria s. elongatus genome recombination i…PromoterLeuP (native S. elongatus promoter) and PrcbLAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3
Plasmid#90087PurposeExpresses wild type caspase 3 in bacterial cellsDepositorAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK4-mC-S
Plasmid#172427PurposeExpression of full-length, human GIRK4 with C-terminal mCherry StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG20
Plasmid#105666Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 20PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG93
Plasmid#105667Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 93PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG233
Plasmid#105838PurposeExpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 233PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-CAts TAG97
Plasmid#105668Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 97PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
HB901: pMVP (L3-L2) polyA
Plasmid#121746PurposepMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertPolyadenylation signal (polyA)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KP201: pMVP (L5-L4) eGFP-P2A
Plasmid#121708PurposepMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.DepositorInserteGFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alanineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alanineAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE
Plasmid#20943Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsmCherryExpressionMammalianMutationH134RPromoterCaMKIIaAvailable SinceJuly 9, 2009AvailabilityAcademic Institutions and Nonprofits only