-
Plasmid#162843PurposeExpression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistanceDepositorInsertProtein Designed Pyocyanin demethylase
UseTagsTEV-cleavable 6x-His tagExpressionBacterialMutationA53N, I73T, A87V, M99V, A129T from WT PodAPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1(L106P)
Plasmid#166714Purposeexpresses AMOT(L106P) in mammalian cellsDepositorInsertAngiomotin p130 L106P (AMOT Human)
UseTags2xHAExpressionMammalianMutationchanged leucine 106 to prolinePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1(A112K)
Plasmid#166715Purposeexpresses AMOT(A112K) in mammalian cellsDepositorInsertAngiomotin p130 A112K (AMOT Human)
UseTags2xHAExpressionMammalianMutationchanged alanine 112 to lysinePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPC-N-Myc-Flag-BirA-hPOT1-DeltaOB
Plasmid#166410Purposeexpress BirA-hPOT1 ΔOB in mammalian cellsDepositorInsertPOT1 ΔOB (POT1 Human)
UseTagsMYC-flag-BirAExpressionMammalianMutationΔOBPromotercmvAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS2155
Plasmid#141065PurposeAAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.DepositorInsertSYN1-EmGFP-WPRE
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceApril 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
pCMV_eA3A T31A-nCas9
Plasmid#163538PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31APromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-nCas9
Plasmid#163564PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCdc20
Plasmid#160954PurposeCdc20 shRNA in pMKO.1 retroviral vectorDepositorInsertCdc20 shRNA (Cdc20 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shKif11
Plasmid#160962PurposeKif11 shRNA in pMKO.1 retroviral vectorDepositorInsertKif11 shRNA (Kif11 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shAspm
Plasmid#160947PurposeAspm shRNA in pMKO.1 retroviral vectorDepositorInsertAspm shRNA (Aspm Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCdkn3
Plasmid#160955PurposeCdkn3 shRNA in pMKO.1 retroviral vectorDepositorInsertCdkn3 shRNA (Cdkn3 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCcnb2
Plasmid#162794PurposeCcnb2 shRNA in pMKO.1 retroviral vectorDepositorInsertCcnb2 shRNA (Ccnb2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y)
Plasmid#155308PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variantDepositorInsertPfAMA1
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationK546M/I547P/I548Y/I549YPromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(M1423R/P1424V)
Plasmid#155309PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationM1423R/P1424V; insert codes for EBA175 residues 1…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424V/Y1425W)
Plasmid#155310PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1425W variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424V/Y1425W; insert codes for EBA175 residues 1…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424V/Y1426H)
Plasmid#155311PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424V/Y1426H; insert codes for EBA175 residues 1…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(Y1426S)
Plasmid#155035PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationY1426S; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424C)
Plasmid#154978PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424C variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424C; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424M)
Plasmid#154979PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424M variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424M; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only