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Plasmid#194557PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > A" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationR > A substitutions after Lys185PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-MUT-Patchless
Plasmid#194553PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44, truncation by stop codon after Lys209PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-CFP-e2a-SOX2-p2a-KLF4
Plasmid#193358PurposeVEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR)DepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterT7Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP5004 pAAV-Syn-DIO-TVA-dTom-SAD-B19G
Plasmid#176286PurposeCre-dependent AAV virusDepositorInsertTVA-dTomato-rabies Glycoprotein
UseTagsdTomatoExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP5005 pAAV-Syn-DIO-TVA66T-dTom-SAD-B19G
Plasmid#176287PurposeCre-dependent AAV virusDepositorInsertTVA66T-dTomato-rabies Glycoprotein
UseTagsdTomatoExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP
Plasmid#176283PurposeRecombinant rabies virus expressing histone-EGFPDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Toronto KnockOut version 3 library (TKOv3)
Pooled Library#90294PurposeOptimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1DepositorAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a G1202R
Plasmid#183830PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationG1202RPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a L1196M/G1202R
Plasmid#183831PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationL1196M/G1202RPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a I1171N/D1203N
Plasmid#183833PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationI1171N/D1203NPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAP1
Plasmid#63674PurposeAccepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.DepositorInsertRFP cloning selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-CFP-e2a-SOX2-17-p2a-KLF4
Plasmid#193360PurposeVEE-CFP-S*K (self-replicating RNA vector expressing CFP + human super-SOX + KLF4 + PuroR) for integration-free naïve conversionDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterT7Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Green monkey (Chlorocebus sabaeus) sgRNA library
Pooled Library#178284PurposeGenome-wide CRISPR screening library targeting 20,991 genes in C. sabaeus, 84,963 total gRNAsDepositorExpressionMammalianSpeciesChlorocebus sabaeus (african green monkey)UseCRISPR and LentiviralAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1
Plasmid#193356PurposeVEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCsDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II
Plasmid#52092PurposeRetroviral expression of all four WT CD3 subunits, co-expressed with EGFPDepositorInsertsUseRetroviralTagsF2A, IRES-EGFP, P2A, and T2AExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II
Plasmid#52093PurposeRetroviral expression of all four WT CD3 subunits, co-expressed with mAmetrineDepositorInsertsUseRetroviralTagsF2A, IRES-EGFP, P2A, and T2AExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 3xFlag
Plasmid#110063PurposeFGFR2 expression in mammalian cells (JCOPCO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 F276C 3xFlag
Plasmid#110064PurposeFGFR2 F276C expression in mammalian cells (JCOPO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Phenylalanine 276 to Cysteine (F276C)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 K41E 3xFlag
Plasmid#110105Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Lysine 41 to Glutamic acid (K41E)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
CN2039-rAAV-3xSP10ins-eHGT_354h-minRho-SYFP2-WPRE3-BGHpA
Plasmid#208401Purposeenhancer AAV for expression of SYFP2 in VIP+ cortical interneuronsInsertSYFP2
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromotereHGT_354h (enhancer)Available sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only