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Showing: 6281 - 6300 of 16077 results

  1. MAPK8_BirA_Cterm

    Plasmid
    #221519
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk8
    Depositor
    Alessandro Ori
    Insert
    MAPK8 (MAPK8 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221519-plasmid-map-sequence-id-439379

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  2. MAPK9_BirA_Cterm

    Plasmid
    #221520
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk9
    Depositor
    Alessandro Ori
    Insert
    MAPK9 (MAPK9 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221520-plasmid-map-sequence-id-439222

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  3. MAPK9_BirA_Nterm

    Plasmid
    #221521
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk9
    Depositor
    Alessandro Ori
    Insert
    MAPK9 (MAPK9 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221521-plasmid-map-sequence-id-439409

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  4. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

    Plasmid
    #171812
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-gB-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171812-plasmid-map-sequence-id-337985

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  5. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid
    #171803
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-gB-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171803-plasmid-map-sequence-id-341955

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  11. pTO_CHMP3_LAP

    Plasmid
    #101849
    Purpose
    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    101849-plasmid-map-sequence-id-200249

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  12. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid
    #114069
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    114069-plasmid-map-sequence-id-220520

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  13. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    114070-plasmid-map-sequence-id-220511

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  15. pDNA2.0 6H-TEV-KRAS G13D

    Plasmid
    #111851
    Purpose
    Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G13D (KRAS Human)
    Use
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 13 to Aspartic Acid
    Promoter
    T5
    Availability
    Academic Institutions and Nonprofits only
    111851-plasmid-map-sequence-id-213465

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  17. pcDNA3-ICUE4

    Plasmid
    #181846
    Purpose
    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    ICUE4 (RAPGEF3 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Glutamine 122 in Epac1 domain mutated to glutamat…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    181846-plasmid-map-sequence-id-364236

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  20. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid
    #217766
    Purpose
    Fluorescent reporter for ceramide (for bacterial expression and purification)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    T7 promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    217766-plasmid-map-sequence-id-435787

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Showing: 6281 - 6300 of 16077 results