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Plasmid#118462PurposecpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.DepositorInsertExRai-AktAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP703-Tubulin
Plasmid#79991PurposeAlpha tubulin labeling with near-infrared fluorescent protein miRFP703DepositorInsertTubulin (TUBA1B Human)
UseTagsmiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
Plasmid#63167PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporterDepositorInsert"spaghetti monster GFP-cMyc"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-Syn21-V5-mCherry-P10
Plasmid#104617PurposeExpresses V5 tagged mCherry upon binding of TALE3 to VAS3DepositorInsertSyn21-V5-mCherry-P10-MhcSA
UseTagsV5ExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
cyto-CFP-FRBx5
Plasmid#103776Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertcyto-CFP-FRBx5
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter
Plasmid#65007PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning siteDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoternoneAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
MMW948: CAG-DmrC-NLS-3xFLAG-VPR
Plasmid#104565PurposeMammalian expression vector for DmrC fused to VPR activatorDepositorInsertDmrC-VPR
UseTags3x FLAG and NLSExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1-Orai3
Plasmid#73563PurposeGreen fluorescent reporter of Orai3-associated calcium influxDepositorInsertG-GECO1-Orai3 (ORAI3 Synthetic, Human)
UseTagsG-GECO1ExpressionMammalianMutationPromoterCMV Immdeiate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9
Plasmid#175523PurposeCo-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertLAMP1-HaloTag11 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…PromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE_Ecad
Plasmid#133805Purposeinducible expression of Ecad in mammalian cellsDepositorInsertHomo sapiens cadherin 1 (CDH1) (CDH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR
Plasmid#118468Purposecp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.DepositorInsertsapphireCKAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-ER-mTagBFP2
Plasmid#216127PurposeLentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum.DepositorInsertEF1a-ProlactinEndoplasmicReticulumSignalSequence-mTagBFP2-KDEL-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-VARNAM2
Plasmid#195520PurposeRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoterDepositorInsertVARNAM2
UseAAVTagsExpressionMammalianMutationVARNAM SI linkerPromoterSynapsinAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR
Plasmid#118472PurposecpBFP-based single-color biosensor for monitoring Protein Kinase C activity.DepositorInsertblueCKAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI
Plasmid#115209PurposeExpresses PbuCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPbuCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTAGBFP2 WC visual barcode
Plasmid#158666Purposetagging clonal populations with whole cell mTAGBFP2 fluorescent proteinDepositorInsertmTAGBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCascade
Plasmid#140622PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNADepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG human DEPTOR
Plasmid#21334DepositorInsertDEPTOR (DEPTOR Human)
UseTagsFLAGExpressionMammalianMutationN204S and S389N mutations (numbering according to…PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-HygR-mCherry
Plasmid#167189PurposeLentiviral expression of sgRNA with hygromycin resistance gene and mCherryDepositorInsertHygromycin B phosphotransferase and mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only