-
Plasmid#68263PurposeLevel 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassetteDepositorInsertPromoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg
Plasmid#60508PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.PI.EGFP.WPRE.bGH (AAV8)
Viral Prep#105530-AAV8PurposeReady-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsEGFPAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-rhMOG
Plasmid#225484PurposeExpress the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coliDepositorInsertSoluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
UseTags6xHis tagExpressionBacterialMutationdeleted amino acids 1-29 (signal peptide) and 150…PromoterT7Available sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule-pCNF
Plasmid#85494PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
APJ-Tango
Plasmid#66224PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertAPJ (APLNR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPLINTR GFP v1
Pooled Library#179774PurposeSPLINTR (Single-cell Profiling and LINeage TRacing) is a high diversity lentiviral barcode library for tracing clonal lineages using bulk and single-cell readouts.DepositorExpressionMammalianUseLentiviralAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-C1
Plasmid#54665PurposeLocalization: C1 Cloning Vector, Excitation: 383, Emission: 448DepositorTypeEmpty backboneUseTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
plRL2
Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Neo/Myc PD-L1
Plasmid#121477Purposeexpression of human Myc-PD-L1 in mamalian cellDepositorInsertCD274 (CD274 Human)
UseTagsMycExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_TIA1
Plasmid#106095Purposebacterial protein expression of TIA1DepositorInsertTIA1 (TIA1 Human)
UseTags6xHIs-thrombin and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mNeptune2.5
Plasmid#197197PurposeExpresses the protein of mNeptune2.5 in mammalian cellsDepositorInsertmNeptune2.5
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMKV059
Plasmid#133314PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPTDepositorInsertLuc+, IPT
UseTagsExpressionPlantMutationPromoterAtUbi10, 35SAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Kir2.1-T2A-tdTomato
Plasmid#60598PurposeExpresses Kir2.1-T2A-tdTomatoDepositorInsertKir2.1-T2A-tdTomato (Kcnj2 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-YHAM-sgRNA_v7
Plasmid#208356PurposeExpression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-YHAM
UseTagsExpressionMammalianMutationF48Y/S188H/V232A/E316MPromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1
Plasmid#222337PurposePlasmid for expressing wild type Bxb1 in the mammalian cellsDepositorInsertCodon-optimized wild type Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlpStop-attB-UAS-2.1-tdTom
Plasmid#88910PurposeattB FlpStop plasmid, for insertion into MiMIC siteDepositorInsertFlpStop
UseTagsExpressionInsectMutationPromoter5XUASAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Plasmid#141395PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInserteGFP
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP
Plasmid#138334PurposeExpresses APOB-Linker-eGFPDepositorInsert5'UTR-ApoB48-Linker-eGFP (APOB Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only