Skip to main content
Addgene
Showing: 6241 - 6260 of 14619 results

  7. pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)

    Plasmid
    #52907
    Purpose
    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Zipp.-L-IFP-F[1]+Plum
    Use
    Retroviral
    Tags
    L-IFP-F[1]
    Expression
    Mammalian
    Mutation
    Promoter
    Unknown
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

    Plasmid
    #172317
    Purpose
    CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    172317-plasmid-map-sequence-id-338787

    Enlarge

  9. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

    Plasmid
    #154779
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    LaG17-SynNotch
    Use
    Tags
    LaG17
    Expression
    Mammalian
    Mutation
    204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154779-plasmid-map-sequence-id-310404

    Enlarge

  14. pX330_CALR sgRNA / hSpCas9

    Plasmid
    #172838
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172838-plasmid-map-sequence-id-340912

    Enlarge

  18. pMT3-RhoGC

    Plasmid
    #100031
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC
    Use
    Tags
    1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)
    Expression
    Mammalian
    Mutation
    Promoter
    Adenovirus major late promoter
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    100031-plasmid-map-sequence-id-192905

    Enlarge

Showing: 6241 - 6260 of 14619 results