Skip to main content
Addgene
Showing: 6221 - 6240 of 9233 results

  1. pFSynW Substance P-pHluorin IRES mRuby3

    Plasmid
    #195699
    Purpose
    Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter
    Depositor
    Edwin Chapman
    Article
    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.
    Insert
    Tac1 (Tac1 Mouse)
    Use
    Lentiviral
    Tags
    pHluorin
    Expression
    Mutation
    Promoter
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195699-plasmid-map-sequence-id-383681

    Enlarge

  2. pDONR P2R-P3-mKate2

    Plasmid
    #48345
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a stop codon at the 3' end of the c…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48345-plasmid-map-sequence-id-70403

    Enlarge

  5. p221k-erTq2CFP

    Plasmid
    #71264
    Purpose
    Entry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    Tq2CFP
    Use
    Gateway
    Tags
    ER retention signal HDEL and signal peptide (ER)…
    Expression
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71264-plasmid-map-sequence-id-134843

    Enlarge

  6. pENTR221-BNT162b2ΔUTR

    Plasmid
    #171214
    Purpose
    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.
    Depositor
    Liang Wei Wang
    Article
    Low et al medRxiv 2021.04.27.21256151
    Insert
    BNT162b2ΔUTR
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Kozak sequence changed from CCCGCCACC to GGCTGCAC…
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171214-plasmid-map-sequence-id-336476

    Enlarge

  8. pBlastR-5xUAS-sfGFP-GMRWhite

    Plasmid
    #165907
    Purpose
    Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable UAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165907-plasmid-map-sequence-id-326309

    Enlarge

  10. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101725-plasmid-map-sequence-id-199888

    Enlarge

  12. pDONRG_P4-P1R:MUM4_0.3Pro_35S

    Plasmid
    #128558
    Purpose
    Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloning
    Depositor
    George Haughn
    Article
    Dean et al Plant Mol Biol. 2017 Sep;95(1-2):33-50.
    Type
    Empty backbone
    Use
    Gateway promoter entry clone
    Tags
    Expression
    Mutation
    Promoter
    MUM4 core promoter with 35S enhancer
    Available since
    Sept. 25, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    128558-plasmid-map-sequence-id-257647

    Enlarge

  14. pDONR P4-P1R-mKate2

    Plasmid
    #48344
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    It contains a Kozak sequence but no stop codon.
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48344-plasmid-map-sequence-id-70388

    Enlarge

  15. pBlastR-5xUAS-sfGFP-MiniWhite

    Plasmid
    #165910
    Purpose
    Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable UAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165910-plasmid-map-sequence-id-329667

    Enlarge

  17. p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid
    #75177
    Purpose
    Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    H2B-mCerulean3-P2A-mKOFP2-CAAX
    Use
    Three prime entry vector for multisite gateway th…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75177-plasmid-map-sequence-id-145999

    Enlarge

Showing: 6221 - 6240 of 9233 results