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Plasmid#101224PurposeFUS(1-214) fused to mCh-Cry2oligDepositorInsertFUS(aa1-214) (FUS Human)
UseLentiviralTagsmCherry-Cry2oligExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-AncBE4max
Plasmid#174164PurposeThis is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.DepositorInsertAncBE4max
UseLentiviralTagsP2A-mCherryExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mouse b3 full length
Plasmid#217814PurposeFull length mouse integrin b3 expressionDepositorInsertITGB3 (Itgb3 Mouse)
UseTagsP2A-mCherryExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-phiC31-LP-TC
Plasmid#183755PurposeAAVS1 targeting construct with the phiC31 landing pad cassette.DepositorInsertFRT-mCherry-attP-BleoR-F3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-TRE-3xFlag-Neurog1-attB53
Plasmid#183613PurposeRMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertNeurog1 (Neurog1 Mouse)
UseRmceTags3xFlagExpressionMammalianMutationPromoterTREAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro
Plasmid#31845DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQ79
Plasmid#175064Purposeexpresses mutant of the huntingtin exon1 in mammalian cellsDepositorInsertpolyQ79 (HTT Human)
UseTagsExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-3
Plasmid#172769PurposeBacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-3
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUW-teto-mch-cFos
Plasmid#193063Purposedoxycycline-inducible expression of mCherry fused to mouse cFos in mammalian cellDepositorInsertmch-cFos
UseLentiviralTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-8
Plasmid#208786PurposeInducibly expresses human Caspase 8 fused to light-activatable Cry2DepositorInsertCaspase 8 (CASP8 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationCARD domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-8
Plasmid#172772PurposeBacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL.Active/Repressed TLR (Sce)
Plasmid#45575PurposeCodes for the Active/Repressed TLR with the target site for I-Sce I nucleaseDepositorInsertseGFP with I-Sce I TS
+3 mCherry
UseLentiviralTagsT2A dislinker and iRFPExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Nucbow
Plasmid#158992PurposeMulticolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-2
Plasmid#172768PurposeBacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh
Plasmid#31847DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherryAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
CSII-PPM1H D288A-mApple
Plasmid#198474PurposeMammalian expression of PPM1H-mAppleDepositorInsertPPM1H-D288A-mApple (PPM1H Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Poly(A)
Plasmid#65525PurposePlasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only