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Plasmid#11107PurposeExpresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
UseTags3x HA and GFPExpressionMammalianMutationContains amino acids 4-460 of NFAT1; L78P compare…PromoterCMVAvailable sinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSL0341 (pTarget)
Plasmid#200906PurposeEncodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.DepositorInsertpTarget
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
Plasmid#196192Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locusDepositorInsert2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
UseZnf donorTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2783 (pTnsC-VP64_Vch)
Plasmid#200896PurposeExpresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.DepositorInsertVchTnsC-VP64
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2669 (pTnsABf_Vch)
Plasmid#200897PurposeExpresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.DepositorInsertVchTnsA-TnsB
UseTagsInternal NLS between TnsA-TnsB fusionExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector
Plasmid#99292PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor
Plasmid#211906PurposeVector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.DepositorInsertDARIC-CD22-BBz
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
UseTagsyeast mitochondrial targeting sequence of citrate…ExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xFlag IFIT1
Plasmid#53554Purposemammalian expression of IFIT1DepositorInsertIFIT1 (IFIT1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-H2AZ-halo
Plasmid#197907Purposesingle-particle tracking for H2AZDepositorInsertH2az2 (H2az2 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v2
Plasmid#113934PurposeAlso termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFPDepositorInsertLOV2 and Stim1 polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
human STIM1 YFP
Plasmid#19754DepositorInsertSTIM1 (STIM1 Human)
UseRetroviralTagsYFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-CaNAR2
Plasmid#64731PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNAR2
UseTagsCerulean3, N-terminal 30 amino acids from DAKAP1,…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-humanTRIM5-FOS
Plasmid#79066PurposeExpress human TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(human)-FOS (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBa.PEX3-tdTomato-FKBP
Plasmid#193941PurposeMammalian expression of tdTomato-tagged PEX3DepositorInsertPEX3 (PEX3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
HSF1-GFPN3
Plasmid#32538DepositorInsertHSF1 (HSF1 Human)
UseTagsEGFPExpressionMammalianMutationsee depositor comments belowPromoterCMVAvailable sinceOct. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xFlag IFIT5
Plasmid#53556Purposemammalian expression of IFIT5DepositorInsertIFIT5 (IFIT5 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-EHER
Plasmid#53262PurposeContains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coliDepositorInsertcrtE, idi, crtX, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_UAS-Ecad
Plasmid#133804Purposeinducible expression of Ecad in mammalian cellsDepositorInsertHomo sapiens cadherin 1 (CDH1) (CDH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only